Journal Title
Title of Journal: Conservation Genet Resour
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Abbravation: Conservation Genetics Resources
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Publisher
Springer Netherlands
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Authors: D B Cunha D Sodré A C A Santos H Schneider I Sampaio F Sequeira M Vallinoto
Publish Date: 2012/10/05
Volume: 5, Issue: 1, Pages: 227-229
Abstract
Toad species Rhinella marina and R schneideri Bufonidae Anura are widely distributed in Amazon and Cerrado/Caatinga respectively Recent data has hypothesized that these two toad species share a history of a massive introgressive hybridization Therefore the aim of the present work was the precise identification of organisms with signs of past introgression or actual ongoing hybridization In this paper we conducted a method based on polymerase chain reaction–restriction fragment length polymorphism through the use of the CfoI enzyme The individuals of R marina have two specific digested bands which is not digested in R schneideri possessing the hybrids three bands The method was very efficient and allowed fast identification of these speciesThis work was partially funded by CAPES Coordenação de Aperfeiçoamento de Pessoal de Nível Superior CNPq Conselho Nacional de Desenvolvimento Científico e Tecnológico and FCT Fundação para a Ciência e a Tecnologia through the research projects MCT/CNPq142010 MCT/CNPq32009 CAPES/FCT244/09 PTDC/BIABEC/105093/2008 and Instituto Nacional de Ciência e Tecnologia INCT em Biodiversidade e Uso da Terra da Amazônia CNPq574008/20080 by postdoctoral grant to FS SFRH /BPD/27134/2006 and by doctoral grants to DBC and DS FAPESPA
Keywords:
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- Development and application of a molecular sexing protocol in the climate change-sensitive American pika
- Development and characterization of 18 microsatellite loci for the spotted salamander ( Ambystoma maculatum ) using paired-end Illumina shotgun sequencing
- Development of microsatellite markers for Adenophora palustris (Campanulaceae), a critically endangered wetland plant species in Japan
- Isolation and characterization of 16 microsatellite markers in Intsia palembanica , a high-value tropical hardwood species
- Development of 16 microsatellite loci for the endangered sandhill dunnart ( Sminthopsis psammophila )
- Fecal samples fail in PCR-based diagnosis of malaria parasite infection in birds
- Isolation and characterization of 12 microsatellite loci from the endemic Schizothorax chongi (Fang, 1936)
- The development of 24 polymorphic microsatellite loci for the endangered barred galaxias, Galaxias fuscus , through next generation DNA sequencing
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- Twenty-nine microsatellite markers for two Australian freshwater turtles, Elseya albagula and Emydura macquarii krefftii : development from 454-sequence data and utility in related taxa
- Isolation and characterization of microsatellite markers for Cichla monoculus (Agassiz, 1831), an important freshwater fish in the Amazon
- Isolation and characterization of twelve novel polymorphic microsatellite loci in the Branchiostoma belcheri Gray (Amphioxus)
- Development of microsatellite markers for the spiny-bellied frog Quasipaa boulengeri (Anura: Dicroglossidae) through transcriptome sequencing
- Development of nine polymorphic microsatellite loci in the squirrel glider ( Petaurus norfolcensis )
- Evaluation of microsatellites for common ungulates in the South African wildlife industry
- Nine microsatellite loci for the Malagasy Poison Frogs ( Mantella )
- Characterization of the complete chloroplast genome of Elaeagnus mollis , a rare and endangered oil plant
- How to use SNPs and other diagnostic diallelic genetic markers to identify the species composition of multi-species hybrids
- Development of 13 polymorphic microsatellite loci for the Greenland Halibut ( Reinhardtius hippoglossoides )
- Environmental DNA detection of redfin perch, Perca fluviatilis
- Development of diploid microsatellite markers for the North American paddlefish ( Polyodon spathula )
- Characterization of 9 polymorphic microsatellite loci in Lamellibrachia sp. 2, a tubeworm found at deep-sea hydrothermal vents and cold seeps
- Development and characterization of twelve polymorphic microsatellite loci for the Chinese concave-eared frog (Odorrana tormota)
- Characterization of ten novel microsatellite markers in Awaous guamensis with comments on cross amplification in congeners and other amphidromous fish native to Hawai’i
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- Thirty-two polymorphic microsatellite loci of the mysid crustacean Mesopodopsis tenuipes
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- Review of the Dra I COI - COII test for the conservation of the black honeybee ( Apis mellifera mellifera )
- Isolation and characterization of 21 polymorphic microsatellite loci in the Japanese dace ( Tribolodon hakonensis )
- Isolation, characterization and PCR multiplexing of polymorphic microsatellite markers in the threatened murine rodent, Leopoldamys neilli
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- Molecular tools reveal diets of insectivorous birds from predator fecal matter
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