Journal Title
Title of Journal: Conservation Genet Resour
|
Abbravation: Conservation Genetics Resources
|
Publisher
Springer Netherlands
|
|
|
|
|
|
Authors: Clayton T Lamb Kelsey M Robson Michael A Russello
Publish Date: 2013/08/23
Volume: 6, Issue: 1, Pages: 17-19
Abstract
Noninvasive sampling paired with molecular sexing provides a valuable tool for the study of elusive mammals yet speciesspecific assays are required to ensure accurate identifications The American pika Ochotona princeps is one example having emerged as a focal mammalian species for studies of metapopulation dynamics and extinction risk in the face of climate change Despite extensive study knowledge of sexspecific patterns has been limited to small sample sizes given the need for livetrapping and fieldbased sexing Here we describe a molecular sexing protocol designed to reliably determine sex from noninvasively collected hair Our polymerase chain reactionbased method coamplifies a malespecific sexdetermining gene Y fragment and a speciesspecific autosomal microsatellite the accuracy of which was validated on 15 internallysexed individuals Subsequent application to 157 hair samples demonstrated high rates of amplification success 960 and unambiguous sex determination 947 revealing a strong malebias in the sample and one instance of inaccurate fieldbased sex identificationJoanna Varner Cheryl Blair and Karl Larsen provided samples of known sex Funding was provided by an NSERC Discovery Grant 341711–07 MR and UBC Okanagan in the form of an Undergraduate Research Award from the Barber School of Arts and Sciences CL and an University Graduate Fellowship KR
Keywords:
.
|
Other Papers In This Journal:
- Development of 44 gene-based SNP markers in Zhikong scallop, Chlamys farreri
- Polymorphic microsatellite loci in the Chinese skink, Plestiodon chinensis (squamata: scincidae)
- Development and characterization of 18 microsatellite loci for the spotted salamander ( Ambystoma maculatum ) using paired-end Illumina shotgun sequencing
- Development of microsatellite markers for Adenophora palustris (Campanulaceae), a critically endangered wetland plant species in Japan
- Isolation and characterization of 16 microsatellite markers in Intsia palembanica , a high-value tropical hardwood species
- Development of 16 microsatellite loci for the endangered sandhill dunnart ( Sminthopsis psammophila )
- Fecal samples fail in PCR-based diagnosis of malaria parasite infection in birds
- Isolation and characterization of 12 microsatellite loci from the endemic Schizothorax chongi (Fang, 1936)
- The development of 24 polymorphic microsatellite loci for the endangered barred galaxias, Galaxias fuscus , through next generation DNA sequencing
- Isolation and characterization of novel microsatellite markers in Gekko gecko (Reptilia: Gekkonidae)
- Twenty-nine microsatellite markers for two Australian freshwater turtles, Elseya albagula and Emydura macquarii krefftii : development from 454-sequence data and utility in related taxa
- Isolation and characterization of microsatellite markers for Cichla monoculus (Agassiz, 1831), an important freshwater fish in the Amazon
- Isolation and characterization of twelve novel polymorphic microsatellite loci in the Branchiostoma belcheri Gray (Amphioxus)
- Development of microsatellite markers for the spiny-bellied frog Quasipaa boulengeri (Anura: Dicroglossidae) through transcriptome sequencing
- Development of nine polymorphic microsatellite loci in the squirrel glider ( Petaurus norfolcensis )
- Evaluation of microsatellites for common ungulates in the South African wildlife industry
- Nine microsatellite loci for the Malagasy Poison Frogs ( Mantella )
- Characterization of the complete chloroplast genome of Elaeagnus mollis , a rare and endangered oil plant
- How to use SNPs and other diagnostic diallelic genetic markers to identify the species composition of multi-species hybrids
- A rapid method for species identification and interspecies introgression based on a PCR–RFLP: application to the closely related Neotropical toads Rhinella marina and R. schneideri (Anura: Bufonidae)
- Development of 13 polymorphic microsatellite loci for the Greenland Halibut ( Reinhardtius hippoglossoides )
- Environmental DNA detection of redfin perch, Perca fluviatilis
- Development of diploid microsatellite markers for the North American paddlefish ( Polyodon spathula )
- Characterization of 9 polymorphic microsatellite loci in Lamellibrachia sp. 2, a tubeworm found at deep-sea hydrothermal vents and cold seeps
- Development and characterization of twelve polymorphic microsatellite loci for the Chinese concave-eared frog (Odorrana tormota)
- Characterization of ten novel microsatellite markers in Awaous guamensis with comments on cross amplification in congeners and other amphidromous fish native to Hawai’i
- Development and characterization of new microsatellite markers for Amur sturgeon ( Acipenser schrenckii)
- A species-specific microsatellite marker to discriminate blue catfish, channel catfish and F1 hybrids
- Eighteen microsatellite loci for the crown-of-thorns starfish, Acanthaster planci
- Development and characterization of polymorphic microsatellite marker for East Asian species of the genus Plestiodon
- Thirty-two polymorphic microsatellite loci of the mysid crustacean Mesopodopsis tenuipes
- Isolation and characterization of 14 microsatellite markers for Rhizophora mucronata (Rhizophoraceae) and their potential use in range-wide population studies
- Twenty-nine polymorphic microsatellite loci in Cyclura carinata , the Turks and Caicos Iguana, a critically endangered island endemic
- Development of polymorphic microsatellite markers in topmouth culter ( Culter alburnus )
- Review of the Dra I COI - COII test for the conservation of the black honeybee ( Apis mellifera mellifera )
- Isolation and characterization of 21 polymorphic microsatellite loci in the Japanese dace ( Tribolodon hakonensis )
- Isolation, characterization and PCR multiplexing of polymorphic microsatellite markers in the threatened murine rodent, Leopoldamys neilli
- Isolation of polymorphic microsatellite markers from amphioxus ( Branchiostoma belcheri ) and a test of cross-species amplification
- Molecular tools reveal diets of insectivorous birds from predator fecal matter
|