Authors: S Ozlem Altinkaya Mustafa Ugur Gulay Ceylaner Mustafa Ozat Tayfun Gungor Serdar Ceylaner
Publish Date: 2009/12/30
Volume: 283, Issue: 2, Pages: 267-272
Abstract
Endometriosis is a chronic gynecological disease characterized by the growth of hormonally responsive endometrial tissue outside the uterine cavity The present study aims to analyze two vascular endothelial growth factor VEGF polymorphisms −460 C/T and +405 C/G in Turkish women with and without endometriosisA case–control study was undertaken at the Infertility Department of Zekai Tahir Burak Women’s Health Care Education and Research Hospital The single nucleotide polymorphisms −460 C/T and +405 C/G in the 5′untranslated region of the VEGF gene were tested in 98 affected women and 94 women with no laparoscopic evidence of disease Endometriosis was also confirmed histologically Following genomic extraction of genomic DNA genotyping of the −460 C/T and +405 C/G polymorphisms of the VEGF gene were performed by polymerase chain reaction and restriction fragment length polymorphism assay Nominal data were evaluated by Pearson Chisquare or Fisher’s Exact test where applicable Odds ratios and 95 confidence intervals were also calculated A P value less than 005 was considered statistically significantDemographic data were similar among groups The genotype and allele frequencies of the −460 C/T polymorphism did not differ significantly between cases and controls In contrast the genotype P 0001 and allele frequencies P 0001 of +405 C/G polymorphism showed a significant difference between cases and controls Regardless of the early or advanced stage women with endometriosis showed a higher incidence of the +405 GC genotype and +405G allele when compared with the controls
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