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Title of Journal: Arch Gynecol Obstet

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Abbravation: Archives of Gynecology and Obstetrics

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Springer Berlin Heidelberg

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DOI

10.1002/chin.200049129

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ISSN

1432-0711

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MiR1246 promotes SiHa cervical cancer cell prolif

Authors: Junying Chen Desheng Yao Shan Zhao Chanjuan He Nan Ding Li Li Fengyi Long
Publish Date: 2014/05/08
Volume: 290, Issue: 4, Pages: 725-732
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Abstract

SiHa cells were assigned into three groups miR1246 analog miR1246 antagonist and control The MTT transwell and wound healing assays were performed to evaluate the proliferation invasion and migration abilities of SiHa cells respectively Western blot was carried out to detect protein expression of thrombospondin2 THBS2 before and after transfection with miR1246 analog antagonist or control In addition a THBS2 3′UTRcontaining dual luciferase plasmid was generated and cotransfected with miR1246 the inhibitor or nonspecific miRNA into SiHa cells to observe its effects on THBS2driven luciferase enzyme activityMTT transwell and wound healing assays revealed that proliferation migration and invasion were all significantly enhanced P  001 in SiHa cells transfected with miR1246 analog but were suppressed in those transfected with the miR1246 antagonist Western blot data showed that miR1246 analogtransfected SiHa cells had significantly decreased THBS2 expression when compared with controltransfected cells gray value = 628 ± 1022 vs 958 ± 1758 P = 0013 while those transfected with the miR1246 antagonist had significantly increased THBS2 expression gray value = 1290 ± 1981 P = 0037 Moreover SiHa cells cotransfected with miR1246 and the THBS2 3′UTRcontaining plasmid exhibited decreased luciferase enzyme activity compared with the controlThe present study was supported by grants from the Natural Science Foundation of Guangxi Zhuang Autonomous Region China 2011GXNSFA018184 2013GXNSFBA019130 2013GXNSFBA019132 wwwgxstinet and the Public Health selffinancing project research project of Guangxi Zhuang Autonomous Region Z2012071

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