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Title of Journal: Arch Gynecol Obstet

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Abbravation: Archives of Gynecology and Obstetrics

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Springer Berlin Heidelberg

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1432-0711

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Simultaneous detection of human papillomavirus int

Authors: Fabrícia Gimenes Raquel Pantarotto Souza André Luelsdorf Pimenta de Abreu Monalisa Wolski Pereira Marcia Edilaine Lopes Consolaro Vânia Ramos Sela da Silva
Publish Date: 2015/08/28
Volume: 293, Issue: 4, Pages: 857-863
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Abstract

The persistence of highrisk oncogenic human papillomavirus HRHPV infection and its integration into the host genome are key steps in the induction of malignant alterations cMYC chromosome region is a frequent localization for HPV insertion that has been observed in chromosome band 8q24 by fluorescence in situ hybridization FISH We report the HPV viral integration and amplification patterns of the cMYC gene in cytological smears with FISH as a potential biomarker for the progression of squamous intraepithelial lesions SILThe results show concordance between FISH and PCR techniques for HPV detection The majority of the samples contained HRHPV the majority being 16 and 18 genotypes HPV integration as determined by FISH was most frequent in highrisk lesions The cMYC gene amplification was found only in HPVpositive samples and was detected primarily in highrisk lesions and in cells with an integrated form of HPVThe author acknowledgements go to Abbott Company for providing the kits for realization of this research and PhD Marcelo Gialluisi Bonini at College of Medicine Departments of Medicine Pharmacology and Pathology University of Illinois at Chicago for invaluable partnership and collaborations This work was supported by grants from Coordenação de Aperfeiçoamento de Pessoal de Nível superior CAPES Brazilian Government PVE A109/2013 and AUX/PEPRODOC 2571/2010

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