Authors: Jianmei Wang Yan Gu Yao Zhang Qian Yang Xuan Zhang Lirong Yin Jian Wang
Publish Date: 2016/02/15
Volume: 293, Issue: 5, Pages: 1125-1135
Abstract
MicroRNAs miRNAs are small noncoding RNA molecules that play critical roles in posttranscriptional gene expression regulation The aim of this study was to identify differentially expressed miRNAs in decidua and villus of recurrent miscarriage RM patientsParticipants were recruited at the outpatient Department of Gynecology and Obstetrics The Second Hospital of Tianjin Medical University China Decidua and villus tissues were collected by curettage from recruited RM patients and normal pregnant women with their informed consent MiRNAs expression profiles in decidua or villus were respectively determined by the deepsequencing analysis The predicated target genes of these differentially expressed miRNAs were analyzed by miRWalk The differential expressions of four miRNAs in decidua and four miRNAs in villus between the six pairs of RM patients and normal pregnant women were confirmed by qRTPCR analysis The expression patterns of two predicated target genes Bcl2 and Pten in the same six pairs of decidual or villus tissues were detected by Western blotting analysis respectivelyTotally 18 RM patients and 15 normal pregnant women were recruited Thirtytwo miRNAs in decidua and four miRNAs in villus of RM patients were screened out to be significantly upregulated compared to that of normal pregnant women and five miRNAs in villus of RM patients were screened out to be remarkably downregulated compared to that of normal pregnant women P value 005 and Fold change 2 These differentially expressed miRNAs were predicted to target a large number of genes that involved in cell apoptosis p53 signaling pathway cell cycle and other cellular biofunctions Differential expressions of hsamiR516a5p 517a3p 519a3p and 519d in decidua as well as hsamiR1 372 1005p and 146a5p in villus were validated by qRTPCR analysis In the decidual of RM patients expression of hsamiR516a5p 517a3p 519a3p and 519d were significantly upregulated compared to normal pregnancy In the villi of RM patients expression of hsamiR100 and 146a5p were significantly higher while hsamiR1 and 372 were significantly lower compared to normal pregnancy Furthermore the expression of Bcl2 and Pten a predicated target gene of hsamiR1 or hsamiR372 respectively was significantly upregulated in the villi of RM patientsThese data suggested that the pathogenic process of RM might be associated with the alteration of miRNAs expression profiles in decidua and villus Especially the aberrant placental expression of hsamiR1 and 372 might be involved in the progression of RM but need to be further investigated by larger studies in the future
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