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Title of Journal: J Cancer Res Clin Oncol

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Abbravation: Journal of Cancer Research and Clinical Oncology

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Springer-Verlag

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DOI

10.1007/s00715-012-0172-2

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1432-1335

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Colour junctions as predictors of radiosensitivity

Authors: Natasja Castro Kreder Chris van Bree Nicolaas A P Franken Jaap Haveman
Publish Date: 2003/08/29
Volume: 129, Issue: 10, Pages: 597-603
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Abstract

Human lung carcinoma cells SW1573 were irradiated with Xrays with or without incubation of 10 nM gemcitabine for 24 h Cell survival was determined with clonogenic assay Colour junctions were measured by whole chromosome FISH of chromosomes 2 and 18 and were scored according to the PAINT methodA clear radiosensitisation by gemcitabine was observed on cell survival A significant decrease in the number of colour junctions was observed after gemcitabine treatment compared with radiation treatment alone The correlation between colour junction induction and cell survival was high for both with and without gemcitabine but the gemcitabinesensitised curve did not coincide with the nonsensitised curve

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