Authors: Hala M Farawela Mervat M Khorshied Neemat M Kassem Heba A Kassem Hamdy M Zawam
Publish Date: 2014/05/08
Volume: 140, Issue: 8, Pages: 1323-1330
Abstract
Multidrug resistance MDR1 represents a major obstacle in the chemotherapeutic treatment of acute leukemia AL Adenosine triphosphate ATPbinding cassette ABCB5 and MDR1 genes are integral membrane proteins belonging to ATPbinding cassette transporters superfamilyThe present work aimed to investigate the impact of ABCB5 and MDR1 genes expression on the response to chemotherapy in a cohort of Egyptian AL patients The study included 90 patients 53 AML cases and 37 ALL cases in addition to 20 healthy volunteers as controlsQuantitative assessment of MDR1 and ABCB5 genes expression was performed by quantitative realtime polymerase chain reaction Additional prognostic molecular markers were determined as internal tandem duplications of the FLT3 gene FLT3ITD and nucleophosmin gene mutation NPM1 for AML cases and mbcrabl fusion transcript for BALL casesIn AML patients ABCB5 and MDR1 expression levels did not differ significantly between de novo and relapsed cases and did not correlate with the overall survival or diseasefree survival AML patients were stratified according to the studied genetic markers and complete remission rate was found to be more prominent in patients having low expression of MDR1 and ABCB5 genes together with mutated NPM1 gene In ALL patients ABCB5 gene expression level was significantly higher in relapsed cases and MDR1 gene expression was significantly higher in patients with resistant disease
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