Authors: Li Xie Xianrang Song Jinming Yu Wei Guo Ling Wei Yanli Liu Xingwu Wang
Publish Date: 2011/09/10
Volume: 137, Issue: 12, Pages: 1739-
Abstract
Two radioresistant subclones A549/R and SPCA1/R derived from lung adenocarcinoma cell lines A549 and SPCA1 were established after eight rounds of sublethal irradiation The new subclones were tested for radioresistant features using clonogenic assay and apoptosis analysis The genes expressed differentially were screened with cDNA microarray analysis consisting of 48000 transcript probes and confirmed by quantitative realtime PCRStable and significant radioresistance was observed in the screened subclones The microarray analysis showed 65 genes were upregulated and 141 genes downregulated in SPCA1/R cells The upregulated and downregulated genes were 708 and 230 in A549/R cells respectively Twentyseven altered genes were consistent in both subclones Interestingly members the of human solute carrier SLC gene superfamily were among in 27 genesThe differentially expressed genes in both cell lines may contribute to their radioresistant phenotype This extensive list of genes identified in the experiment provides a large body of potentially valuable information for studying the molecular mechanisms of radiosensitivity and identification of candidate molecular markers of radiation sensitivity Thus to our knowledge the SLC gene superfamily is the first being reported to involve in acquired radioresistance
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