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Title of Journal: J Cancer Res Clin Oncol

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Abbravation: Journal of Cancer Research and Clinical Oncology

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Springer-Verlag

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DOI

10.1007/BF03294150

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1432-1335

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Isolation and comparison of mesenchymal stemlike

Authors: Xiaomeng Xu Xu Zhang Sheng Wang Hui Qian Wei Zhu Huiling Cao Mei Wang Yuan Chen Wenrong Xu
Publish Date: 2010/05/15
Volume: 137, Issue: 3, Pages: 495-504
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Abstract

Mesenchymal stem cell MSClike cells a subpopulation of tumor microenvironment have been isolated from several tumor tissues In our previous study we identified MSClike cells in human gastric cancer tissues and found their characteristics to be similar to those of bone marrow MSCs However whether there are MSClike cells in adjacent noncancerous tissues and any difference between the MSClike cells derived from tumor and noncancerous tissues are not clear The aim of this study is to study and research the differences of two mesenchymal stemlike cellsOur results revealed that hGCMSCs and hGCNMSCs possessed similar morphological properties stem cellassociated gene expression as well as multipotential differentiation capability We also found differences in cell surface markers pluripotency and proliferationrelated gene expression between hGCNMSCs and hGCMSCsWe thank Dr Ruiwen Zhang Comprehensive Cancer Center and Gene Therapy Center University of Alabama School of Medicine Birmingham USA for assistance in editing this manuscript This work was supported by the National Natural Science Foundation of China grant no 30471983 the Natural Science Foundation of the Ministry of Public Health of China grant no WKJ20052024 Jiangsu Province’s Outstanding Medical Academic Leader Program LJ200614 and the Natural Science Foundation of the Ministry of Education of the Jiangsu Province grant no 06KJD310040Neither the submitted paper Isolation and comparison of mesenchymal stemlike cells from human gastric cancer and adjacent noncancerous tissues nor any similar paper either in whole or in part has been or will be submitted to or published in any other primary scientific journal All of the authors are aware of and agree to the content of the paper and their being listed as author on the paper There are no financial or other interests with regard to the paper that represent a conflict of interest

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