Authors: Cabezas Gloria A Tabernero Ester Romero Eduardo Velasco Manuel
Publish Date: 2004/05/01
Volume: 17, Issue: S1, Pages: 209A-209A
Abstract
Gloria A Cabezas Ester Tabernero Eduardo Romero Manuel Velasco P481 Dopaminergic dilatation on cholinergic and electric induced contractions of rat isolated tracheal muscle American Journal of Hypertension Volume 17 Issue S1 May 2004 Page 209A https//doiorg/101016/jamjhyper200403555We have previously demostrated in humans that inhaled dopamine 051 μg/ml/min induces bronchodilatation in subjects suffering acute asthma attack but did not modify the resting bronchial tone in normal subjects or in asthmatics without acute bronchospasm Dopamine is likely to exert its inhibitory action on bronchial muscle by stimulating adrenergic or dopaminergic receptors The present work examined the possible direct effect of dopamine on the airways smooth muscle of SragueDawley rats Tracheal rings were excised and placed in an organ bath containing Ringers solutionat 37aC gassed with oxygen 95 and carbon dioxide 5 Contractile responses were recorded with an isometric transducer in a polygraph Letical Spain Contraction was induced by electric field stimulation or pharmacologically by adding acetylcholine into the bath Dopamine cumulative dose/response/curves were obtained Dopamine inhibited both elecrical and cholinergic induced tracheal contractions Dopaminergic dilatation was not blocked by propranolol 01μM or terazosin 10μM administration 30 minutes before contractions were elicited Phenylephrine 010μM did not reversed terazosin blocking action These results suggest that dopamine inhibition is mediated by dopaminergic receptors rather than by β2 or α1 adrenergic receptor activation
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