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Title of Journal: Pharm Res

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Abbravation: Pharmaceutical Research

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Springer US

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DOI

10.1002/adma.200890015

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1573-904X

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Global Gene Expression Profiling in Cultured Cells

Authors: Tatsuaki Tagami Kiyomi Hirose Jose Mario Barichello Tatsuhiro Ishida Hiroshi Kiwada
Publish Date: 2008/06/26
Volume: 25, Issue: 11, Pages: 2497-2504
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Abstract

We investigated global gene expression in a HT1080 cell line by a cDNA microarray We also evaluated the effect of lipofection on global gene expression by determining the change of the expression of an exogenous gene green fluorescence protein GFP and also determined treatmentrelated cytotoxicityTreatment of the cells with either siRNAlipoplexes or cationic liposomes altered the expression of approximately 2500 genes When lipoplexes containing nonspecific siRNAs were used GFP expression was enhanced In this case the effect was independent on the dose and type of siRNA in the formulation By contrast when lipoplexes containing a specific siRNA against GFP was used GFP expression was markedly diminished These results clearly indicate that an efficient reduction of a targeted gene expression by a specific siRNA is accompanied by a significant alteration of the expression of numerous nontargeted genes In addition treatmentrelated cytotoxicity increased with siRNA and cationic lipiddoses but was not dependent on siRNA type

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