Authors: Patrizia Rosignoli Roberto Fabiani Angelo De Bartolomeo Raffaela Fuccelli Maria Antonietta Pelli Guido Morozzi
Publish Date: 2008/08/06
Volume: 47, Issue: 6, Pages: 301-309
Abstract
Colorectal cancer is the second cause of death for tumour worldwide Among the risk factors for this disease the dietary habits seem to have a pivotal role An elevated intake of fats causes a high release in the gut lumen of bile acids that are positively correlated with colorectal cancer since they act as detergents and proliferation promoters Recently it was evidenced that bile acids can also be able to induce DNA damageIn this study the genotoxicity of deoxycholic acid DCA and chenodeoxycholic acid CDCA has been evaluated in human normal colonocytes derived from 60 colon biopsies and in tumour cells The involvement of reactive oxygen species ROS and the oxidative DNA damage was assessed In addition the protective effect exerted by both two wellknown antioxidants commonly present in the diet βcarotene and αtocopherol and butyrate which is known to be involved in the regulation of several cellular functions has also been testedBile acids CDA and CDCA resulted genotoxic on both normal and tumour human colon cells The inclusion of the endonuclease III digestion step in the comet assay demonstrated that bile acids induced an oxidative DNA damage In addition treatment of colonocytes with bile acids in the presence of the antioxidants βcarotene αtocopherol and Nabutyrate caused a reduction of DNA damageWe are very grateful to Dr A Solinas and Dr F Farroni for their competent technical assistance in the endoscopical evaluation and biopsies sampling We would particularly thank Drssa M R Mantovani Cucchia Administrator and Dr M Badolato Medical Director respe of Clinica Porta Sole in Perugia Italy to put the structure at our disposal
Keywords: