Authors: Maria G Kakkoura Christiana A Demetriou Maria A Loizidou Giorgos Loucaides Ioanna Neophytou Simon Malas Kyriacos Kyriacou Andreas Hadjisavvas
Publish Date: 2015/07/01
Volume: 55, Issue: 4, Pages: 1535-1544
Abstract
Oxidative stress arises due to a cellular imbalance in oxidants and antioxidants and/or due to an altered activity of antioxidant enzymes caused by SNPs Oxidative stress increases susceptibility to breast cancer BC risk and we previously showed that the Mediterranean diet MD which is rich in antioxidants reduces BC risk in GreekCypriot women Here we investigated the effect of MnSOD pVal16Ala rs4880 and CAT 262CT rs1001179 SNPs on the association between the MD and BC risk in the case–control study of BC MASTOS in CyprusDietary intake data were obtained using a 32item food frequency questionnaire from which a dietary pattern was previously derived using principal component analysis This pattern included high loadings of vegetables fruit legumes and fish a combination that closely resembles the MD and was used as our dietary variableHigh vegetable intake lowered BC risk in women with at least one MnSOD Val allele ORHigh vs Low for Val/Val = 056 95 CI 035–088 for Val/Ala = 057 95 CI 039–082 or one CAT 262C allele ORHigh vs Low for 262CC = 066 95 CI 047–092 for 262CT = 053 95 CI 035–081 High fish intake conferred a decreased BC risk of CAT 262CC women ORQ4 vs Q1 066 95 CI 047–092 compared with the CAT 262TT women and low fish intake ORQ2 vs Q1 279 95 CI 108–717 Additionally high fish intake reduced BC risk in MnSOD Val/Val women ORQ4 vs Q1 063 95 CI 040–098 p interaction values were however not statistically significantThis work was supported by “Cyprus Research Promotion Foundation” grants 0104/13 0104/17 and the Cyprus Institute of Neurology and Genetics Maria G Kakkoura is funded by the Eurobank Cyprus Scholarship provided through the Cyprus School of Molecular Medicine Further we would like to thank all the study participants and acknowledge the help of the following cancer patient’s organisations the Pancyprian Association of Cancer Patients and Friends Europa Donna Cyprus the Cyprus Anticancer Society as well as Dr Vaios Partasides director of the National breast cancer screening programme and his team We also would like to express our appreciation to Doctors Eleni Kakouri Panayiotis Papadopoulos Maria Daniel and Yiola Marcou as well as to all the nurses and volunteers who provided valuable help towards the recruitment of the study participantsThe study was approved by the Cyprus National Bioethics Committee All procedures followed were in accordance with the ethical standards of the responsible committee on human experimentation institutional and national and with the Declaration of Helsinki 1975 as revised in 2000 Informed consent was obtained from all patients for being included in the study
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