Authors: Thereza Cristina Lonzetti Bargut Anna Carolina Alves Gomes SilvaeSilva Vanessa SouzaMello Carlos Alberto MandarimdeLacerda Marcia Barbosa Aguila
Publish Date: 2015/01/23
Volume: 55, Issue: 1, Pages: 159-169
Abstract
Fish oil FO elicits diverse beneficial effects Reduction in or prevention of body mass BM gain in animal models may be associated with modulation of brown adipose tissue BAT We aimed to evaluate the effects of different highfat diets with FO on BAT metabolism and thermogenic markersC57BL/6 male mice 3monthold were fed different diets during 8 weeks standardchow diet SC 10 fat highfat lard diet HFL 50 fat highfat lard plus FO diet HFL+FO 50 fat and highfat FO diet HFFO 50 fat We evaluated BM and performed an oral glucose tolerance test At euthanasia plasma was collected for leptin and triacylglycerol measurement and interscapular BAT was dissected and stored for molecular analysesHFL group showed elevated BM glucose intolerance associated with diminished TC10 and GLUT4 expressions hypertriglyceridemia associated with increased CD36 and diminished CPT1 expression elevated expression of proinflammatory cytokines and reduced PPAR expression Furthermore these animals showed hyperleptinemia with increased expression of thermogenic markers beta3AR PGC1alpha and UCP1 Conversely HFL+FO and HFFO groups showed reduced BM gain with regularization of glucose tolerance and triglyceridemia GLUT4 TC10 CD36 CPT1 and cytokines expressions Both groups exhibited elevated PPAR and thermogenic markers expression in a dosedependent wayThe authors would like to thank Mrs Thatiany Marinho Mrs Aline Penna Mrs Gezileia Lau and Mrs Michele Soares for their technical assistance This study was supported by Conselho Nacional de Desenvolvimento Científico e Tecnológico CNPq Fundação de Amparo a Pesquisa do Estado do Rio de Janeiro FAPERJ and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior CAPES Brazil
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