Authors: Jie Luo Jing Feng Jun Lu Yuping Wang Xuanbin Tang Fei Xie Wenbin Li
Publish Date: 2009/08/14
Volume: 27, Issue: 3, Pages: 791-797
Abstract
To study the relation of 1433 sigma gene promoter hypermethylation and its transcription expression levels in sporadic breast carcinogenesis Methylation of 1433 sigma gene was detected by sensitive MSP assay in carcinous noncancerous and normal tissue and its mRNA was also detected by real time PCR based on SYBR Green 1 as well and protein was detected by west blotting assay The methylation frequencies of 1433 sigma were 90 in 68 cases of sporadic breast cancer patients Methylation was presented in portions 2/13 18 of hyperplastic samples and no hypermethylation was presented in normal tissue The methylation change of 1433 sigma gene was markedly related with various types grades and lymph node metastases P 005 and no significant differences in methylation frequencies were seen between premenopause and postmenopause P 005 The methylation of 1433 sigma shows reverse relation with its mRNA transcription and expression level P 005 Only was lymph node metastases strongly associated with poor outcome P = 002 Whether 1433 sigma promoter methylation or not did not affect the 5 years survival rate of sporadic breast cancer patients P 005 Epigenetics alterations of the 1433 sigma can contribute to reducing or losing expression of 1433 sigma protein which play an important role in the development of sporadic breast carcinomas and involved in various types grades and lymph node metastases Otherwise node metastases of breast carcinogenesis patients are strongly associated with poor outcomeThis study was funded by the Natural Science Funds of Hubei Province No 2007ABA371 the Hubei Provincial Department of Education NoQ20082407 Natural Science Funds for Distinguished Young Scholar of Hubei Province No2008154 and Innovation Term for Young Scholar of Yunyang Medical College NoCXX200805
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