Authors: Milena Krajnović Milica Radojković Radoslav Davidović Bogomir Dimitrijević Koviljka Krtolica
Publish Date: 2012/12/30
Volume: 30, Issue: 1, Pages: 441-
Abstract
In this study methylationspecific polymerase chain reaction was used to investigate the role and potential prognostic significance of the methylation status of p16 p15 MGMT and DAPK genes in 32 specimens of follicular lymphoma FL Hypermethylation of p15 gene was associated with lower hemoglobin level P = 0020 and MGMT/DAPK comethylation with relapsed disease P = 0018 Among all patients with FL there was no significant difference in the overall survival between those with hypermethylated and unmethylated of any examined genes Therefore we analyzed methylation in the different groups according to FL International Prognostic Index FLIPI and tumor grade In the highrisk group patients with hypermethylated p16 gene had significant lower overall survival than those with unmethylated p16 P = 0006 and trend toward shorter failurefree survival P = 0068 In the same risk group there was a trend toward longer overall survival for patients with hypermethylated MGMT gene compared to those with unmethylated MGMT gene P = 0066 p15 methylation had impact on shorter overall survival in grade I group of patients P = 0013 and DAPK methylation tended to have impact on shorter failurefree survival in the whole examined group P = 0079 Our results suggest that promoter methylation of p16 and MGMT genes could have prognostic value when used in combination with the FLIPI and p15 methylation in combination with tumor grade Concurrent methylation of MGMT and DAPK genes could be the marker of tumor chemoresistance and disease recurrence
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