Dietinduced obesity and prenatal undernutrition l

Authors: Mhoyra Fraser Charisma K Dhaliwal Mark H Vickers Stefan O Krechowec Bernhard H Breier

Publish Date: 2016/03/16

Volume: 53, Issue: 3, Pages: 839-847

PDF Link

Abstract

Previously we reported that prenatal undernutrition UN leads to a dysregulation of appetite suppression through alterations in hypothalamic neuropeptide gene expression In the current study we expand our observations and investigate neuroendocrine transcriptional responses and central leptin sensitivity within the arcuate nucleus of rats exposed to prenatal UN or a postnatal highfat diet HF Pregnant Wistar rats were fed a standard chow diet either ad libitum AD or at 30  of AD intake throughout gestation UN resulting in either control or intrauterine growthrestricted female offspring At weaning AD offspring were fed either a chow C or a HF 30  fat wt/wt diet ad libitum for the remainder of the study whereas UN offspring were fed a chow diet only At ~142 days AD and UN offspring received either recombinant rat leptin L or saline S subcutaneously for 14 days Prenatal UN had a significant effect on hypothalamic NPY P  00001 AgRP P  001 and ObRb P  002 mRNA expression compared to AD chowfed offspring A postnatal HF diet had a significant effect on AgRP mRNA expression P  0001 compared to AD chowfed offspring but no effect on NPY and ObRb expression Leptin treatment in both UN and HF offspring was ineffective in reducing NPY and AgRP mRNA expression and had no effect on ObRb expression These findings suggest that prenatal UN and a postnatal HF diet lead to differential neuroendocrine gene expression in the hypothalamic arcuate nuclei and reduced sensitivity to leptin’s anorexigenic effectsBHB and MF wrote the grant applications for funding support for the studies BHB SK and MV conceptualised and designed all in vivo experimental procedures and research strategies MF conceptualised the design and implementation of all molecular studies MF and CKW performed all molecular expression analyses MF and CKW analysed all molecular data MF BHB SK and MV provided critical interpretation of the results MF drafted the manuscript MF BHB SF and MV edited and revised the manuscript All authors have read and approved the final version of the manuscriptThe authors have no conflicts of interest to disclose The study sponsors had no role in 1 study design 2 the collection analysis and interpretation of data 3 the writing of the report and 4 the decision to submit the manuscript for publication No honorarium grant or other form of payment was given to anyone to produce the manuscript

Keywords: