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Title of Journal: Trans R Soc Trop Med Hyg

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Abbravation: Transactions of The Royal Society of Tropical Medicine and Hygiene

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Narnia

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DOI

10.1016/0142-9612(85)90083-3

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0035-9203

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Recovery of infective Schistosoma mansoni schistos

Authors: James Eric R Farrant John
Publish Date: 1977/01/01
Volume: 71, Issue: 6, Pages: 498-500
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Abstract

Eric R James John Farrant Recovery of infective Schistosoma mansoni schistosomula from liquid nitrogen A step towards storage of a live schistosomiasis vaccine Transactions of The Royal Society of Tropical Medicine and Hygiene Volume 71 Issue 6 1977 Pages 498–500 https//doiorg/101016/0035920377901432Successful recovery of infective schistosomula of Schistosoma mansoni following storage at −196 °C is reported The technique involves a twostep cooling procedure—slow cooling 065 °C min−1 to an intermediate temperature of −28 °C followed by rapid cooling into liquid nitrogen 10000 °C min−1 Rewarming 10000 °C min−1 and rapid dilution to remove the cryoprotectant 175 methanol yielded motile organisms some of which developed to adult worms in mice after intramuscular injection The percentage of schistosomula developing to adult worms was small 044 but is a significant step towards storage of trematode larvae and of a live attenuated vaccine for schistosomiasis

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References

citation title=Ultrastructural appearance of freezesubstituted lymphocytes frozen by interrupting rapid cooling with a period at −26 °C Cryobiology citation year=1975 citation volume=12 citation pages=103109

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