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Title of Journal: Trans R Soc Trop Med Hyg

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Abbravation: Transactions of The Royal Society of Tropical Medicine and Hygiene

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Narnia

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DOI

10.1007/bf02502110

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0035-9203

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Immunogenicity of axenic Entamoeba histolytica ant

Authors: Sawhney Sunita Chakravarti RN Jain Parmeel Vinayak VK
Publish Date: 1980/01/01
Volume: 74, Issue: 1, Pages: 26-29
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Abstract

Sunita Sawhney RN Chakravarti Parmeel Jain VK Vinayak Immunogenicity of axenic Entamoeba histolytica antigen and its fractions Transactions of The Royal Society of Tropical Medicine and Hygiene Volume 74 Issue 1 1980 Pages 26–29 https//doiorg/101016/0035920380900061Amoebic antigen prepared from axenic Entamoeba histolytica NIH200 strain grown at 37 °C in TPS I medium was chromatographed on a Sephadex G200 column Three fractions FI FII FIII were obtained the molecular weights of which ranged from 650000 to 1450 Fractions were concentrated by lyophilization and antisera against each fraction and whole antigen were raised in rabbits Partially purified fractions were used in various serological tests and their reactivities were compared with whole amoebic antigen Main haemagglutinating and precipitating activities were confined to Fraction I which had 14 of total protein and the highest molecular weight 650000 5 μg/ml of protein contained in this fraction gave antibody titres comparable to those obtained with whole antigen with 40μg/ml of protein Other fractions although they had precipitin activity lacked haemagglutinating activity The protective efficacies of these fractions are being investigated in our laboratory

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References

citation title=Immunoelectrophoresis test in amoebiasis Indian Journal of Medical Research citation year=1976 citation volume=64 citation pages=661667

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