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Title of Journal: Trans R Soc Trop Med Hyg

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Abbravation: Transactions of The Royal Society of Tropical Medicine and Hygiene

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Narnia

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DOI

10.1016/0301-6226(93)90148-b

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0035-9203

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A rapid in vitro assay system using antibromodeox

Authors: Doi Hiroyuki Ishii Akira Shimono Kunio
Publish Date: 1988/03/01
Volume: 82, Issue: 2, Pages: 190-193
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Abstract

Hiroyuki Doi Akira Ishii Kunio Shimono A rapid in vitro assay system using antibromodeoxyuridine for drug susceptibility of Plasmodium falciparum Transactions of The Royal Society of Tropical Medicine and Hygiene Volume 82 Issue 2 MarchApril 1988 Pages 190–193 https//doiorg/101016/003592038890404XDrug effects were monitored by the measurement of bromodeoxyuridine BrdU incorporation into parasite deoxyribonucleic acid DNA Cells are pulselabelled with BrdU and those which are synthesizing DNA incorporate BrdU into their DNA AntiBrdU is used to identify cells undergoing DNA synthesis at the time of the pulse Concentrationeffect curves were determined for chloroquine pyrimethamine and quinine in culture for only 3 h in RPMI 1640 medium supplemented with BrdU Different strains of Plasmodium falciparum could be distinguished when BrdU uptake was monitored by the ELISA This assay offers a fast and accurate method of monitoring the effects of a variety of antimalarial drugs

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References

citation title=Stagedependent effects of chloroquine on Plasmodium falciparum in vitro Journal of Protozoology citation year=1983 citation volume=30 citation pages=642647

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