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Title of Journal: Planta

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Abbravation: Planta

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Springer-Verlag

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10.1016/0167-4838(82)90248-5

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1432-2048

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Integrinlike protein at the invaginated plasma me

Authors: Minako Sakurai JunYong Pak Yuki Muramatsu Toshiyuki Fukuhara
Publish Date: 2004/08/17
Volume: 220, Issue: 2, Pages: 271-277
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Abstract

By immunoblotting with antihuman integrin β polyclonal antibodies β1 β3 or β5 a single distinct band of about 60 kDa was detected in total protein extracts from mature leaves of the seagrass Zostera marina L but no band was detected in total protein extracts from immature seagrass leaves freshwater grass leaves or Arabidopsis thaliana L Heynh leaves This integrinlike protein was detected by indirect immunofluorescence microscopy on the surface of nonspherical protoplasts of epidermal cells isolated from mature seagrass leaves using an antiintegrin β3 polyclonal antibody Electronmicroscopic analyses with the same antibody indicated that this integrinlike protein was localized specifically in the invaginated plasma membrane of epidermal cells in mature seagrass leaves Therefore this integrinlike protein of about 60 kDa may be involved in the developmentally regulated invagination of the plasma membrane in epidermal cells of the seagrass Z marinaWe thank Dr S Takagi of Osaka University for his useful suggestions and valuable advice This research was supported in part by grants from the Salt Science Research Foundation No 9822 and the Tokyu Foundation No 207 to TF and by a Sasakawa Scientific Research Grant No 14372MK to YM from the Japan Science Society

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