Decreased ROS level and activation of antioxidant

Authors: Y N Shkryl G N Veremeichik V P Bulgakov T Y Gorpenchenko D L Aminin Y N Zhuravlev

Publish Date: 2010/08/03

Volume: 232, Issue: 5, Pages: 1023-1032

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Abstract

Microbe–plant interactions often lead to a decrease in the reactive oxygen species ROS level of plant cells which allows pathogen survival through the suppression of plant immune responses In the present investigation we tested whether transformation of Rubia cordifolia cells by Agrobacterium rhizogenes had a similar effect We isolated partial cDNA sequences of ascorbate peroxidase catalase and Cu/Zn superoxide dismutase genes RcApx1 RcApx2 RcApx3 RcCAT1 RcCAT2 RcCSD1 RcCSD2 and RcCSD3 from plant tissues as well as pRiA4transformed and normal calli of Rubia cordifolia and studied their expression by realtime PCR Transcription profiling revealed that ascorbate peroxidase RcApx1 and Cu/Zn superoxide dismutase RcCSD1 were the most abundant transcripts present in both plant tissues and nontransformed calli Catalase genes were weakly expressed in these samples The pRiA4transformed calli showed enhanced expression of several genes encoding ROSdetoxifying enzymes Confocal microscopy imaging revealed decreased ROS level in pRiA4transformed calli compared to the control These results demonstrate that A rhizogenes like other plant pathogens uses a strategy aimed at decreasing ROS levels in host cells through the general upregulation of its antioxidant genesThis work was supported by grants from the Russian Foundation for Basic Research by the Grant Program “Molecular and Cell Biology” of the Russian Academy of Sciences and by grants from the Far East Branch of the Russian Academy of Sciences 09IIIA06187 10IIIB06086 09IIIA05137 The authors would like to express their gratitude to Dr S Shedko Dr E Artyukova Dr M Kozyrenko and Dr A Gontcharov for their help with phylogenetic analysisSuppl Fig 1 Maximumlikelihood cladogram for ascorbate peroxidase isoenzymes obtained using Phyml program 300 bootstraps Similar tree was also obtained with the maximum parsimony method from Phylip package 1000 bootstraps Numbers on the branches represent bootstrap values as obtained from ML/MP respectively only values 50 are presented lower values are denoted ‘’ The GenBank accession numbers of the proteins are given in the cladogram The sequences obtained in this work are marked by dots JPG 204 kbSuppl Fig 2 Maximumlikelihood cladogram for Cu/Zn superoxide dismutase isoenzymes obtained using Phyml program 300 bootstraps Similar tree was also obtained with the maximum parsimony method from Phylip package 1000 bootstraps Numbers on the branches represent bootstrap values as obtained from ML/MP respectively only values 50 are presented lower values are denoted ‘’ The GenBank accession numbers of the proteins are given in the cladogram The sequences obtained in this work are marked by dots JPG 146 kbSuppl Fig 3 Maximumlikelihood cladogram for catalase isoenzymes obtained using Phyml program 300 bootstraps Similar tree was also obtained with the maximum parsimony method from Phylip package 1000 bootstraps Numbers on the branches represent bootstrap values as obtained from ML/MP respectively only values 50 are presented lower values are denoted ‘’ The GenBank accession numbers of the proteins are given in the cladogram The sequences obtained in this work are marked by dots JPG 144 kb

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