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Title of Journal: Planta

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Abbravation: Planta

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Springer Berlin Heidelberg

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DOI

10.1007/bf00712459

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ISSN

1432-2048

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DNA barcodes for the identification of Emphasis T

Authors: Tongwei Lv Renda Teng Qingsong Shao Hongzhen Wang Wangshu Zhang Mingyan Li Lili Zhang
Publish Date: 2015/06/24
Volume: 242, Issue: 5, Pages: 1167-1174
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Abstract

Chinese medicinal herbs have a similar appearance and are easily confused complicating identification via traditional methods This study provided a scientific approach based on DNA barcoding to accurately and rapidly identify Anoectochilus roxburghii and its adulterants This technology complements traditional methods of identification of medicinal herbsA comparison of the DNA barcodes matK psbAtrnH and ITS2 was performed to verify that the ITS2 sequence is an effective marker for rapidly and accurately identifying A roxburghii and its closely related species Genomic DNA extracted from A roxburghii and its adulterants were used as templates and the ITS2 sequence was amplified using PCR amplification and sequencing Species identification was conducted using BLAST1 and neighborjoining trees The 12 samples were successfully classified into four species based on the ITS2 sequence The ITS2 sequence length of A roxburghii was 253 bp The average intraspecific genetic distance of A roxburghii was 00021 markedly lower than the interspecific genetic distance between A roxburghii and its adulterants 00380 Our findings illustrate that ITS2 sequence can accurately and efficiently distinguish A roxburghii and its adulterants In addition the results provided reference for molecular identification of other Chinese herbal medicineThis work was supported by the National Natural Science Foundation of China Grant No 81303167 the Zhejiang Provincial Natural Science Foundation Grant No LY13H280010 LY14H280008 Zhejiang Province Public Technology Applied Research Projects Grant No 2014C32017 Ningbo Agricultural Science and Technology Research Projects Grant No 2014C10013 and Science and Technology Innovation Program for Students of Zhejiang Xinmiao Talent Scheme 2015R412003 We thank the anonymous reviewers for carefully reviewing our manuscript and making many valuable suggestions

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