Authors: Junxiang Zhang Hui Yuan Zhangjun Fei Barry J Pogson Lugang Zhang Li Li
Publish Date: 2015/02/17
Volume: 241, Issue: 6, Pages: 1381-1394
Abstract
The orange head phenotype of Br or resulted from a large insertion in carotenoid isomerase BrCRTISO Comparative transcriptome analysis revealed that the mutation affected the expression of abundant transcription factor genes A new orange traitspecific marker was developed for markerassisted breedingOrange head leaves are a desirable quality trait for Chinese cabbage Our previous fine mapping identified BrCRTISO as the Bror candidate gene for the orange Chinese cabbage mutant Here we examined the BrCRTISO gene from white and orange head Chinese cabbage While BrCRTISO from the white control plant was able to complement the Arabidopsis Atcrtiso mutant phenotype Brcrtiso with a large insertion from the orange head Chinese cabbage failed to rescue the Arabidopsis mutant phenotype The results show that Brcrtiso was nonfunctional concomitant with the accumulation of prolycopene in Bror to yield orange head Comparative transcriptome analysis by RNAseq identified 372 differentially expressed genes between the control and Bror mutant using two nearisogenic lines with white and orange inner leaves The mutation in BrCRTISO specifically affected many genes in the functional groups involved in RNA protein transport and signaling Particularly expressions of many transcription factor genes were dramatically altered in Bror suggesting a potential role of BrCRTISO or carotenoid metabolites in affecting transcription A novel codominant genespecific marker was developed that cosegregated with orange color phenotype and would be useful for markerassisted selection with enhanced selection efficiency Our study provides new insights into understanding of the molecular basis of Bror in mediating head leaf color and depicts a global view of the effect of BrCRTISO on cellular processes in plant It also provides a molecular tool to accelerate breeding new Chinese cabbage cultivars with unique health quality and visual appearance
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