Authors: Ann Moons
Publish Date: 2008/09/17
Volume: 229, Issue: 1, Pages: 53-71
Abstract
The role of plant pleiotropic drug resistance PDR type ATPbinding cassette ABC transporters remains poorly understood We characterized the expression of the rice pleiotropic drug resistance PDR gene family in roots where PDR transporters are believed to have major functions A prototypical oligonucleotide array was developed containing 70mers chosen in the genespecific 3′ untranslated regions of the rice PDR genes other fullmolecule rice ABC transporter genes and relevant marker genes Jasmonates which are involved in plant defense and secondary metabolism proved major inducers of PDR gene expression Over half of the PDR genes were JAinduced in roots of rice OsPDR9 to the highest level Salicylic acid involved in plant pathogen defense markedly induced the expression of OsPDR20 OsPDR20 was cDNA cloned and characterized Abscisic acid typically involved in water deficit responses particularly induced OsPDR3 in roots and shoot and OsPDR6 in rice leaves OsPDR9 and OsPDR20 were furthermore upregulated in response to dithiothreitol or glutathioneinduced redox perturbations Exogenous application of the weak organic acids lactic acid malic acid and citric acid differentially induced the expression of OsPDR3 OsPDR8 OsPDR9 and OsPDR20 in rice seedling roots This transcriptional survey represents a guide for the further functional analysis of individual PDR transporters in roots of rice
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