Authors: Freddy Boutrot Donaldo Meynard Emmanuel Guiderdoni Philippe Joudrier MarieFrançoise Gautier
Publish Date: 2006/09/16
Volume: 225, Issue: 4, Pages: 843-862
Abstract
Plant nonspecific lipid transfer proteins nsLTPs are encoded by a multigene family and support physiological functions which remain unclear We adapted an efficient ligationmediated polymerase chain reaction LMPCR procedure that enabled isolation of 22 novel Triticum aestivum nsLtp TaLtp genes encoding types 1 and 2 nsLTPs A phylogenetic tree clustered the wheat nsLTPs into ten subfamilies comprising 1–7 members We also studied the activity of four type 1 and two type 2 TaLtp gene promoters in transgenic rice using the βGlucuronidase reporter gene The activities of the six promoters displayed both overlapping and distinct features in rice In vegetative organs these promoters were active in leaves and root vascular tissues while no βGlucuronidase GUS activity was detected in stems In flowers the GUS activity driven by the TaLtp72a TaLtp91a TaLtp92d and TaLtp93e gene promoters was associated with vascular tissues in glumes and in the extremities of anther filaments whereas only the TaLtp94a gene promoter was active in anther epidermal cells In developing grains GUS activity and GUS immunolocalization data evidenced complex patterns of activity of the TaLtp71a TaLtp92d and TaLtp94a gene promoters in embryo scutellum and in the grain epicarp cell layer In contrast GUS activity driven by TaLtp72a TaLtp91a and TaLtp93e promoters was restricted to the vascular bundle of the embryo scutellum This diversity of TaLtp gene promoter activity supports the hypothesis that the encoded TaLTPs possess distinct functions in plantaThe authors wish to thank Caroline Hartman for the wheat genomic library Julie Petit for the CaMV35SuidA construct Jacques Escoute and Geneviève Conejero for helpful advice on histological analysis We would also like to thank Emmanuelle Bourgeois for help in adapting the LMPCR protocol to wheat Freddy Boutrot was the recipient of a fellowship from the French Ministère de l’Education Nationale de l’Enseignement Supérieur et de la Recherche The support of the Génopole LR for containment greenhouse infrastructures is also acknowledged
Keywords: