Authors: J A Ocaña González M Callejón Mochón F J Barragán de la Rosa
Publish Date: 2005/08/22
Volume: 151, Issue: 1-2, Pages: 39-45
Abstract
A liquid chromatographic method with a C18 column and acetonitrile/01 M phosphoric acid/ sodium hydroxide buffer pH 30/001 M noctylamine pH 30 as mobile phase in gradient mode has been developed and optimised for the simultaneous determination of the cephalosporin cefepime and the quinolones garenoxacin levofloxacin and moxifloxacin Identification and quantification was carried out with a diodearray UV detector with working wavelengths of 256 nm for cefepime 292 nm for levofloxacin 294 nm for moxifloxacin and 282 nm for garenoxacin The mobile flowrate and sample volume injected were 1 mL min−1 and 20 µL respectively The retention times and detection limits for each antibiotic were 49 min and 19 µg mL−1 for cefepime 75 min and 22 µg mL−1 for levofloxacin 89 min and 27 µg mL−1 for moxifloxacin and 107 min and 18 µg mL−1 for garenoxacin respectively The method was applied to the determination of the four molecules in spiked samples of human urine
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