Authors: Xiujuan Chen Yukun Huang Xiaoyuan Ma Fei Jia Xiaofei Guo Zhouping Wang
Publish Date: 2015/05/01
Volume: 182, Issue: 9-10, Pages: 1709-1714
Abstract
We are presenting an aptasensor for the sensitive determination of fumonisin B1 FB1 via electrochemical impedance spectroscopy EIS and applying aptamerbased biorecognition A thiolated aptamer for FB1 was anchored onto the surface of gold nanoparticles AuNPs on a glassy carbon electrode A significant increase in resistance Ret is found on interaction with FB1 in the 01 nM to 100 μM concentration range and the detection limit is as low as 2 pM The assay was applied to determine FB1 in spiked maize samples and gave recovery rates ranging from 91 to 105 The results demonstrate this method to present new possibilities in the application of aptamers in food safety analysisThe electrodeposited AuNPs create a nanomaterial platform in situ for anchoring of FB1 aptamers serving as recognition elements The change in EIS signals induced by aptamertarget interactions was measured and a significant increase in resistance Ret is found on interaction with FB1 in the 01 nM to 100 μM concentration range
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