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Title of Journal: Microchim Acta

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Abbravation: Microchimica Acta

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Springer Vienna

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DOI

10.1007/bf01967196

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1436-5073

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A chemiluminescence assay for Lhistidine based on

Authors: Xu Hun Yaqiong Xu Li Bai
Publish Date: 2014/09/14
Volume: 182, Issue: 3-4, Pages: 565-570
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Abstract

We describe a chemiluminescence CL assay for Lhistidine that is based on the use of DNAzyme covalently immobilized on 15μm sized magnetic beads On addition of a substrate labeled with a CL reagent the DNAzyme and substrate form a stable duplex by allosteric synergetic stabilization of each duplex If Lhistidine is added to this system selfcleavage of the substrate occurs through catalytic reaction and results in the formation of two fragments which dissociate from the beads After removal of the magnetic beads the labeled fragments can be detected by CL whose intensity is linearly related to the concentration of Lhistidine in the 10 to 1000 nM range The detection limit is 03 nM and the RSD is 34  at a 50 nM level n = 9 The method has been successfully applied to the determination of Lhistidine in spiked human serum samples and holds promise as a widely applicable general platform for DNAzymebased CL detection of small organic molecules and of metal ionsThis research was supported by the National Natural Science Foundation of China 21005045 21275085 the Open Project Program of State Key Laboratory of Food Science and Technology Jiangnan University SKLFKF201112 the Scientific and Technical Development Project of Qingdao 1214318jch the Scientific Research Startup Foundation of Qingdao University of Science and Technology for Talents

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