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Title of Journal: Microchim Acta

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Abbravation: Microchimica Acta

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Springer-Verlag

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DOI

10.1007/s002200050438

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1436-5073

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Biochemical sniffer with choline oxidase for measu

Authors: Hiroyuki Kudo Teruyoshi Goto Takao Saito Hirokazu Saito Kimio Otsuka Kohji Mitsubayashi
Publish Date: 2007/07/09
Volume: 160, Issue: 4, Pages: 421-426
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Abstract

An enzymebased gas sensor biosniffer for choline vapour was fabricated and tested The biosniffer was constructed using a Clarktype dissolved oxygen electrode and an enzyme choline oxidase immobilized membrane This bioelectronic device measures choline concentration by the oxygen consumption induced by an enzyme reaction of choline oxidase As the assessment of sensor performances the calibration curves for choline in the liquid and gas phases were investigated respectively The responses of the bioelectronic device to choline solutions of various concentrations were related within a range from 500 to 700 µmol·L−1 with a correlation coefficient of 0999 On the other hand the biosniffer for choline vapour was placed into a gasmeasuring chamber and calibrated using gas detection tubes The calibration range was 100–300 ppm correlation coefficient 0996 The response time for choline vapour was approximately 15 slower than that of biosensor for choline solution These results indicate that the biosniffer is useful to monitor colourless and odourless choline gas released from coating compositions including choline

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