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Title of Journal: Microchim Acta

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Abbravation: Microchimica Acta

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Springer Vienna

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DOI

10.1002/jpln.19600900133

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1436-5073

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Colorimetric chiral discrimination and determinati

Authors: Cunwei Liu Baoxin Li Chunli Xu
Publish Date: 2014/05/18
Volume: 181, Issue: 11-12, Pages: 1407-1413
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Abstract

We report on a method for visual differentiation between the D and Lforms of tryptophan Trp It is based on the inherent chirality of citratecapped silver nanoparticles AgNPs ca 15 nm in diameter that can be used as chiral selector for D and Lforms of Trp On addition of DTrp to a solution of the AgNPs a color change from yellow to red can be seen while no color change is found on addition of LTrp The chiral assay can be used to determine the enantiometric excess of DTrp in the range from −50  to 100  The effect can be detected with bare eyes and quantified by spectrophotometry Notably this method does not require any labeling or chiral modification The method excels by its sensitivity low cost good availability of materials and its simplicity

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