Authors: Fenfen Liang Chenyao Liu Jiao Jiao Shuoqi Li Ji Xia Jingbo Hu
Publish Date: 2012/03/09
Volume: 177, Issue: 3-4, Pages: 389-395
Abstract
We report on a simple strategy for the fabrication of gold nanoparticles AuNPs on an indium tin oxide substrate using a modified ion implantation method The morphology structure and electrochemical features of AuNPs were characterized by atomic force microscopy electrochemical impedance spectroscopy and cyclic voltammetry The modified electrode has a large electrochemically active surface and enables strong loading with cytochrome c Cyt c proteins It undergoes enhanced electron transfer at uncompromised electrochemical activity and also displays good stability and repeatability The immobilized Cyt c exhibits good electrocatalytic activity towards hydrogen peroxide H2O2 with a linear relationship between the catalytic current during differential pulse voltammetry and the concentration of H2O2 in the 005 μM to 02 μM range The detection limit S/N = 3 is 001 μMA biosensor for H2O2 was developed by immobilizing cytochrome c on ITO electrode modified by gold ion implantation process Ion implantation is a new type and an useful simply strategy to attach gold ions on the electrode The biosensor exhibited a low detection limit good reproducibility and stability which indicated that ionimplanted gold nanoparticles could construct enzyme electrode for biosensor applicationThe authors wish to express their gratitude and appreciation for the financial support from the National Natural Science Foundation of China No20211130505 and the Key Laboratory of Beam Technology and Material Modification of Ministry of Education China
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