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Title of Journal: Microchim Acta

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Abbravation: Microchimica Acta

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Springer Vienna

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DOI

10.1007/bf01894673

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1436-5073

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Direct electrochemistry of myoglobin based on elec

Authors: Jinmei Huang Jianbin Zheng Qinglin Sheng
Publish Date: 2011/01/13
Volume: 173, Issue: 1-2, Pages: 157-163
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Abstract

We report on the direct electrochemistry and electrocatalytic properties of myoglobin Mb immobilized on a carbon ionic liquid electrode covered with a matrix composed of an ionic liquid gellan gum and Pd nanoparticles UVvis and FTIR spectroscopy confirm that Mb retains its native structure in the composite film on the electrode Scanning electron microscopy reveals that the nanoparticles are deposited on the surface of the Pd electrode Cyclic voltametry gives a pair of welldefined and quasireversible redox peaks with a formal potential E 0′ of −332 mV and a peaktopeak separation of 64 mV at nearneutral pH value The modified electrode shows good electrocatalytic activity towards the reduction of hydrogen peroxide with a linear range between 50 μM and 027 mM and a detection limit of 17 μM S/N = 3 The apparent MichaelisMenten constant is 88 μMThis work was supported by the National Natural Science Foundation of China No 20875076 the Scientific Initializing Foundation of Northwest University No PR09036 the Science Foundation of Northwest University No 09NW02 and the Northwest University Doctorate Dissertation of Excellence Funds No 09YYB02

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