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Title of Journal: Microchim Acta

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Abbravation: Microchimica Acta

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Springer Vienna

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DOI

10.1007/s11007-007-9060-7

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1436-5073

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Glucometerbased quantitative determination of Hg

Authors: Jin Zhang Ying Tang Jing Lv Shuangqiang Fang Dianping Tang
Publish Date: 2014/12/07
Volume: 182, Issue: 5-6, Pages: 1153-1159
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Abstract

We report on a simple and sensitive protocol for quantitative assay of HgII ions The process involves the following steps A thymine Trich singlestranded DNA1 is used to modify the surface of a well of a microplate by the use of biotinavidin interface chemistry In parallel clusters of gold microspheres with a core containing the enzyme invertase were prepared and modified via thiol chemistry with a second Trich oligomer DNA2 If the gold clusters incorporating invertase and carrying DNA2 are added to the DNA1 immobilized in the wells of the microplate no interaction will occur and the gold cluster will be removed in the subsequent washing step If however HgII is present in the sample the DNA on the gold clusters incorporating the enzyme invertase will bind to the DNA in the wells due to the formation of strong THgIIT links between the two DNA strands The encapsulated invertase will not be removed in the following washing step Sucrose is added in the next step along with invertase which will hydrolyze it to form glucose and fructose The quantity of glucose formed increases with the quantity of HgII ions present in the sample The glucose generated is then quantified by using a commercial personal glucometer Under optimal conditions the signal for glucose increases with HgII concentration in the range from 005 to 80 nM and the detection limit is as low as 10 pM The assay has good repeatability and shows an intermediate precision of down to 75  The method is highly specific for HgII over other metal ions It was applied to the determination of HgII in naturally contaminated sewage and in spiked samples of drinking water This approach has a wide scope of application in that it may be extended to numerous other kinds of nanoparticles oligomer interactions enzymes and ionsSupport by the National Natural Science Foundation of China grant nos 41176079 21475025 the National Science Foundation of Fujian Province grant no 2014 J0105 and the Program for Changjiang Scholars and Innovative Research Team in University grant no IRT1116 is gratefully acknowledged

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