Paper Search Console

Home Search Page About Contact

Journal Title

Title of Journal: Microchim Acta

Search In Journal Title:

Abbravation: Microchimica Acta

Search In Journal Abbravation:

Publisher

Springer Vienna

Search In Publisher:

DOI

10.1007/s11007-007-9060-7

Search In DOI:

ISSN

1436-5073

Search In ISSN:
Search In Title Of Papers:

Glucometerbased quantitative determination of Hg

Authors: Jin Zhang Ying Tang Jing Lv Shuangqiang Fang Dianping Tang
Publish Date: 2014/12/07
Volume: 182, Issue: 5-6, Pages: 1153-1159
PDF Link

Abstract

We report on a simple and sensitive protocol for quantitative assay of HgII ions The process involves the following steps A thymine Trich singlestranded DNA1 is used to modify the surface of a well of a microplate by the use of biotinavidin interface chemistry In parallel clusters of gold microspheres with a core containing the enzyme invertase were prepared and modified via thiol chemistry with a second Trich oligomer DNA2 If the gold clusters incorporating invertase and carrying DNA2 are added to the DNA1 immobilized in the wells of the microplate no interaction will occur and the gold cluster will be removed in the subsequent washing step If however HgII is present in the sample the DNA on the gold clusters incorporating the enzyme invertase will bind to the DNA in the wells due to the formation of strong THgIIT links between the two DNA strands The encapsulated invertase will not be removed in the following washing step Sucrose is added in the next step along with invertase which will hydrolyze it to form glucose and fructose The quantity of glucose formed increases with the quantity of HgII ions present in the sample The glucose generated is then quantified by using a commercial personal glucometer Under optimal conditions the signal for glucose increases with HgII concentration in the range from 005 to 80 nM and the detection limit is as low as 10 pM The assay has good repeatability and shows an intermediate precision of down to 75  The method is highly specific for HgII over other metal ions It was applied to the determination of HgII in naturally contaminated sewage and in spiked samples of drinking water This approach has a wide scope of application in that it may be extended to numerous other kinds of nanoparticles oligomer interactions enzymes and ionsSupport by the National Natural Science Foundation of China grant nos 41176079 21475025 the National Science Foundation of Fujian Province grant no 2014 J0105 and the Program for Changjiang Scholars and Innovative Research Team in University grant no IRT1116 is gratefully acknowledged


Keywords:

References


.
Search In Abstract Of Papers:
Other Papers In This Journal:

  1. Horseradish peroxidase-loaded nanospheres attached to hollow gold nanoparticles as signal enhancers in an ultrasensitive immunoassay for alpha-fetoprotein
  2. Comparison of Dynamic Simulations with RBS Measurements of Low Energy Ion Implantation of Sb + into SiO 2 /Si Substrates
  3. A fluorescent chemical sensor for ethanol determination in alcoholic beverages
  4. Click chemistry-based synthesis of water-dispersible hydrophobic magnetic nanoparticles for use in solid phase extraction of non-steroidal anti-inflammatory drugs
  5. A glassy carbon electrode modified with the nickel(II)-bis(1,10-phenanthroline) complex and multi-walled carbon nanotubes, and its use as a sensor for ascorbic acid
  6. Non-enzymatic sensing of glucose and hydrogen peroxide using a glassy carbon electrode modified with a nanocomposite consisting of nanoporous copper, carbon black and nafion
  7. Dynamic evaluation of cell-secreted interferon gamma in response to drug stimulation via a sensitive electro-chemiluminescence immunosensor based on a glassy carbon electrode modified with graphene oxide, polyaniline nanofibers, magnetic beads, and gold nanoparticles
  8. Ultrasensitive Assay of Rhein in Medicine Based on its Enhanced Luminol-K 3 Fe(CN) 6 Chemiluminescence Reaction Using the Flow Injection Technique
  9. Image Formation in Low Vacuum SEM
  10. Chemometrics to evaluate the quality of water sources for human consumption
  11. Schnellbestimmung von Hexachlorcyklohexan neben DDT mit Hilfe der Mikrobrechungsindexbestimmung nach Kofler
  12. Colorimetric and bare-eye detection of alkaline earth metal ions based on the aggregation of silver nanoparticles functionalized with thioglycolic acid
  13. An on-site immunosensor for ractopamine based on a personal glucose meter and using magnetic β-cyclodextrin-coated nanoparticles for enrichment, and an invertase-labeled nanogold probe for signal amplification
  14. Determination of alkaloids by a ring colorimetry method
  15. A Pre-Concentration Procedure Using Cloud Point Extraction for the Determination of Manganese in Saline Effluents of a Petroleum Refinery by Flame Atomic Absorption Spectrometry
  16. Magnetic Separation Immunoassay for Digoxin in Plasma with Flow Injection Fluorescence Detection
  17. Graphene nanocomposite modified glassy carbon electrode for voltammetric determination of the antipsychotic quetiapine
  18. Direct electrochemistry of myoglobin based on electrodeposition of Pd nanoparticles with carbon ionic liquid electrode as basic electrode
  19. Direct electrochemistry of myoglobin based on electrodeposition of Pd nanoparticles with carbon ionic liquid electrode as basic electrode
  20. A chemiluminescence assay for L-histidine based on controlled DNAzyme catalytic reactions on magnetic microparticles
  21. Coupling Cloud Point Extraction to Instrumental Detection Systems for Metal Analysis
  22. Die Zersetzung des Hexacyanoferrates(II) in der Analyse. III
  23. Formaldehyde-sensitive conductometric sensors based on commercial and recombinant formaldehyde dehydrogenase
  24. Miniaturized module with biosensors for potentiometric determination of urea
  25. The Corrected Value of the Y La Mass Absorption Coefficient in Silicon
  26. Adsorptive Voltammetric Study of Thorium–Alizarin Complexon Complex at a Carbon Paste Electrode
  27. Ionic liquid-based headspace single-drop microextraction coupled to gas chromatography for the determination of chlorobenzene derivatives
  28. The reduction and determination of small amounts of rhenium-VII
  29. New polyacrylate-based lead(II) ion-selective electrodes
  30. Solvent extraction production of high specific activity 59 Fe and carrier free 54 Mn from irradiated iron targets
  31. Microextraction by packed sorbent coupled with gas chromatography—mass spectrometry: application to the determination of metabolites of monoterpenes in small volumes of human urine
  32. Spectres infra-rouge des gels de savons métalliques dans les liquides organiques
  33. Monolithic solid-phase extraction for the rapid on-line monitoring of microcystins in surface waters
  34. Impedimetric aptamer-based determination of the mold toxin fumonisin B1
  35. Application of L-Cysteine-Capped ZnS Nanoparticles in the Determination of Nucleic Acids Using the Resonance Light Scattering Method
  36. Trans-membrane electron transfer in red blood cells immobilized in a chitosan film on a glassy carbon electrode
  37. Microdetermination of sulfur amino acids with N-bromosuccinimide using bordeaux red as an indicator
  38. Colorimetric determination of neomycin using melamine modified gold nanoparticles
  39. New poly(aryleneethynylene)s as optical active platforms in biosensing. Selective fluorescent detection of Hg(II) obtained by the use of aminoacidic groups anchored on conjugated backbones
  40. Biochemical sniffer with choline oxidase for measurement of choline vapour
  41. The study of preconcentration and separation of vanadium(V) using microcrystalline triphenylmethane loaded with crystal violet and the determination of vanadium(V) in water samples by spectrophotometry
  42. Determination of total boron in sea water by atomic absorption spectroscopy
  43. ITO electrode modified by a gold ion implantation technique for direct electrocatalytic sensing of hydrogen peroxide
  44. Flow Injection Chemiluminescence for the Determination of Estriol via a Noncompetitive Enzyme Immunoassay
  45. Über eine halbautomatische mikrogravimetrische Schnellmethode zur Bestimmung von Kohlenstoff in organischen Verbindungen
  46. Epitope imprinted polymer nanoparticles containing fluorescent quantum dots for specific recognition of human serum albumin
  47. Giant magneto-resistance based immunoassay for the tumor marker carcinoembryonic antigen
  48. Orientation Imaging in Scanning Electron and Transmission Electron Microscopy for Characterization of the Shear Banding Phenomenon
  49. Development of an acetylspiramycin sensor based on a single-walled carbon nanotubes film electrode
  50. Calixarenes for Biochemical Recognition and Separation
  51. Fabrication of Fe 3 O 4 @graphene oxide core-shell nanospheres for ferrofluid-based dispersive solid phase extraction as exemplified for Cd(II) as a model analyte
  52. Über die Mikrobestimmung von Chlor, Brom und Jod im Sauerstoffstrom
  53. Zur mikroanalytischen Bestimmung von Phosphor in Ribo- und Desoxyribonukleinsäuren
  54. Sensing epinephrine with an ITO electrode modified with an imprinted chitosan film containing multi-walled carbon nanotubes and a polymerized ionic liquid
  55. Biosensor for aluminium(III) based on its inhibition of α-chymotrypsin immobilized on a screen-printed carbon electrode modified with gold nanoparticles
  56. Biosensor for aluminium(III) based on its inhibition of α-chymotrypsin immobilized on a screen-printed carbon electrode modified with gold nanoparticles
  57. Colorimetric chiral discrimination and determination of enantiometric excess of D/L-tryptophan using silver nanoparticles
  58. The electrocatalytic activity of a supramolecular assembly of CoTsPc/FeT4MPyP on multi-walled carbon nanotubes towards L-glutathione, and its determination in human erythrocytes
  59. Rapid determination of melamine in milk and milk powder by surface-enhanced Raman spectroscopy and using cyclodextrin-decorated silver nanoparticles
  60. Piezoelectric atrazine sensor based on a molecularly imprinted film of titanium dioxide
  61. Platinum-Group Element Distribution in Some Ore Deposits: Results of EPMA and Micro-PIXE Analyses
  62. Double-template synthesis of platinum nanomaterials for oxygen reduction
  63. Electrochromatography in the separation of ions
  64. Arsenic Speciation Analysis in Solutions Treated with Zeolites
  65. Electrochemical determination of hesperidin using mesoporous SiO 2 modified electrode
  66. Simultaneous voltammetric determination of dopamine and uric acid using carbon-encapsulated hollow Fe 3 O 4 nanoparticles anchored to an electrode modified with nanosheets of reduced graphene oxide
  67. Examination of the Plenum and Deposition Coupon of the Phebus FPT4 Test by Scanning Electron Microscopy and Photoemission Spectroscopy
  68. New Equation for the Evaluation of Standard Addition Experiments Applied to Ion Chromatography
  69. Voltammetric determination of the endocrine disruptor diethylstilbestrol by using a glassy carbon electrode modified with a composite consisting of platinum nanoparticles and multiwalled carbon nanotubes
  70. A novel anti-interference and pH-modulation device: application to enzyme-free glucose detection
  71. Reduced graphene oxide conjugate thymine as a new probe for ultrasensitive and selective fluorometric determination of mercury(II) ions
  72. Electrical communication between electrode and dehydrogenase by a ferrocene-labeled high molecular-weight cofactor derivative: application to a reagentless biosensor
  73. Sensitive Adsorption Stripping Voltammetric Determination of Reserpine by a Glassy Carbon Electrode Modified with Multi-Wall Carbon Nanotubes
  74. Sensitive Adsorption Stripping Voltammetric Determination of Reserpine by a Glassy Carbon Electrode Modified with Multi-Wall Carbon Nanotubes
  75. Spectrographic analysis of brass alloys
  76. Interrogation of immunoassay platforms by SERS and SECM after enzyme-catalyzed deposition of silver nanoparticles
  77. Pyrolytic graphite electrode modified with a thin film of a graphite/diamond nano-mixture for highly sensitive voltammetric determination of tryptophan and 5-hydroxytryptophan
  78. Selective assembly of Au-Fe 3 O 4 nanoparticle hetero-dimers
  79. Separation and purification of hydrophobic magnetite-gold hybrid nanoparticles by multiphase density gradient centrifugation
  80. Titanium dioxide nanorod-based amperometric sensor for highly sensitive enzymatic detection of hydrogen peroxide
  81. Novel chemiluminescent assay for staphylococcal enterotoxin B
  82. Simultaneous Determination of Cefepime and the Quinolones Garenoxacin, Moxifloxacin and Levofloxacin in Human Urine by HPLC-UV
  83. Miniaturized and green method for determination of chemical oxygen demand using UV-induced oxidation with hydrogen peroxide and single drop microextraction
  84. Determination of traces of copper and silver in tantalum metal by anodic stripping voltammetry
  85. Microfluidic system for enzymeless electrochemical determination of inulin using catalytically active metal nanowires
  86. Spectrophotometric Determination of Copper in Waste Water Using Liquid–Liquid Extraction in a Flow-Injection System

Search Result: