Authors: Si Chen Jiabin Zhang Ning Gan Futao Hu Tianhua Li Yuting Cao Daodong Pan
Publish Date: 2014/10/25
Volume: 182, Issue: 3-4, Pages: 815-822
Abstract
We report on an onsite ultrasensitive sandwich immunoassay for ractopamine RAC that is based on the use of a personal glucose meter RAC is preconcentrated with the help of magnetic nanoparticles coated with βcyclodextrin Fe3O4SiO2CD and a novel polymer tag labeled with invertase is employed to catalyze the hydrolysis of sucrose to form glucose which is detected with the glucose meter Invertasemodified gold nanoparticles were prepared and immobilized along with antibodies against RAC on an Envision reagent a polymer that contains many antiIgG antibodies Because of its high loading with the enzyme the resulting copolymers strongly amplify the signal The concentration of RAC is calculated on the basis of the linear relationship between the concentration of RAC and the amount of glucose formed The method exhibits a detection limit of 034 μg kg−1 as determined for a sample of pork This onsite testing method may also be applied to the detection of other lowmolecular toxins by simply changing the antibodyRactopamine is preconcentrated with the help of magnetic nanoparticles coated with βcyclodextrin Fe3O4SiO2CD and a novel polymer tag labeled with invertase is employed to recognize analyte and catalyze the hydrolysis of sucrose to form glucose which is detected with the glucose meterThis work was supported by the National Natural Science Foundation of China Nos 31070866 the Science and Technology Project of China 2012BAK08B012 the Natural Science Foundation of Zhejiang LY13C200017 LY12B01005 the Natural Science Foundation of Ningbo 2013A610241 2013A610163 2014A610184 the Science and Technology Project of Zhejiang 2013C37033 2013A610157 2012C23101 and 2011C31037 and the KC Wong Magna Fund in Ningbo University
Keywords: