Authors: Qian Li Kui Cheng Wenjian Weng Piyi Du Gaorong Han
Publish Date: 2013/10/03
Volume: 180, Issue: 15-16, Pages: 1487-1493
Abstract
Titanium dioxide nanorods TNR were grown on a titanium electrode by a hydrothermal route and further employed as a supporting matrix for the immobilization of nafioncoated horseradish peroxidase HRP The strong electrostatic interaction between HRP and TNR favors the adsorption of HRP and facilitates direct electron transfer on the electrode The electrocatalytic activity towards hydrogen peroxide H2O2 was investigated via cyclic voltammetry and amperometry The biosensor exhibits fast response a high sensitivity 4169 μA·mM−1 a wide linear response range 25 nM to 046 mM a detection limit as low as 12 nM and a small apparent MichaelisMenten constant 336 μM The results indicate that this method is a promising technique for enzyme immobilization and for the fabrication of electrochemical biosensorsA TiO2 nanorod film was directly grown on Ti substrate by a hydrothermal route and was further employed for a supporting matrix to immobilize horseradish peroxidase as a biosensor electrode The asprepared hydrogen peroxide biosensor based on Nafion/HRP/TNR/Ti electrode exhibited fast response and excellent electrocatalytic activity toward H2O2 ie a high sensitivity 4169 μA mM−1 a wide linear range 25 × 10−8 to 46 × 10−4 M with a low detection limit 0012 μM and a small apparent MichaelisMenten constant 336 μM
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