Authors: Erbao Liu Bingchun Xue
Publish Date: 2005/12/30
Volume: 153, Issue: 1-2, Pages: 51-56
Abstract
Determination of the effective components in traditional Chinese medicine is one of the key steps for its identification In this paper a novel and sensitive chemiluminescence CL method for the determination of rhein coupled with flowinjection analysis FIA is developed It is based on the strong sensitizing effect on the weak CL reaction between luminol and ferricyanide in alkaline solution Under optimal experimental conditions the relative CL intensity is proportional to the concentration of rhein in the range of 70 × 10−12–70 × 10−10 mol L−1 and 10 × 10−9–40 × 10−5 mol L−1 the detection limit is 1478 × 10−13 mol L−1 and the relative standard deviation RSD for 9 parallel measurements of 1408 × 10−7 mol L−1 rhein is 34 The method was successfully applied to the determination of rhein in pharmaceutical preparations The possible mechanism of CL is also briefly discussed
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