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Title of Journal: Microchim Acta

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Abbravation: Microchimica Acta

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Springer Vienna

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DOI

10.1007/s11896-016-9193-x

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1436-5073

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Colorimetric determination of neomycin using melam

Authors: Can Xiao Junfeng Liu Ankang Yang Hong Zhao Yujian He Xiangjun Li Zhuobin Yuan
Publish Date: 2015/03/28
Volume: 182, Issue: 7-8, Pages: 1501-1507
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Abstract

The colorimetric assay for neomycin presented here is based on melaminemodified gold nanoparticles melAuNPs and the finding that hydrogen bonding between melamine and neomycin results in the aggregation of melAuNPs This results in a change in the color of the solution from wine red to blue and in a redshift of the absorption maximum of the melAuNPs The concentration of neomycin can be determined by spectrophotometry The ratio of absorptions at 680 nm and 520 nm is linearly related to the logarithm of the concentration of neomycin in the 01 to 50 nM range and in the 5 to 100 nM range with regression coefficients of 0997 and 0999 respectively The detection limit at an S/N ratio of 3 is 30 pM This is far below the usual safety limit The method was applied to the detection of trace levels of neomycin in milk samples and gave recoveries between 98 and 105 

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