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Title of Journal: Microchim Acta

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Abbravation: Microchimica Acta

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Springer Vienna

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DOI

10.1016/0167-9317(94)00069-7

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1436-5073

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Interrogation of immunoassay platforms by SERS and

Authors: Felipe Conzuelo Stefanie Grützke Lutz Stratmann José M Pingarrón Wolfgang Schuhmann
Publish Date: 2015/09/24
Volume: 183, Issue: 1, Pages: 281-287
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Abstract

The manuscript demonstrates the interrogation of immunoassay platforms after enzymecatalyzed deposition of silver nanoparticles AgNPs using scanning electrochemical microscopy SECM and surfaceenhanced Raman scattering SERS We have selected an immunoassay model platform for the determination of residues of the antibiotic sulfapyridine in milk The assay involves a direct competitive approach with an HRPlabeled antigen analog and selective capture antibodies immobilized on the surface of glassy carbon plates modified with protein G Interrogation of the modified substrate by SECM and SERS was carried out after enzymecatalyzed insitu deposition of AgNPs The enhanced Raman scattering for proflavine in the presence of AgNPs was used as signalling system HexacyanoferrateIII was applied as the redox probe in SECM the visualization of the deposited AgNP spots was enabled by using a competition between the SECM tip microelectrode and the modified carbon plate for the oxidation of ferrocyanide This SECM strategy proved to be an efficient tool for the interrogation of sensing surfaces that were amplified by enzymecatalyzed silver depositionScanning electrochemical microscopy SECM image of different spots on a modified glassy carbon plate prepared after incubation with solutions of variable sulfapyridine SPY concentration in the presence of a fixed amount of SPYHRP and further enzymecatalyzed silver particles depositionThe Cluster of Excellence RESOLV EXC 1069 funded by the Deutsche Forschungsgemeinschaft is acknowledged for financial support The authors are grateful to Prof MPilar Marco Nb4D group Barcelona Spain for providing the reagents used for sulfonamide immunoassay F C acknowledges a FPU fellowship from the Spanish Ministry of Education

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