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Title of Journal: Microchim Acta

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Abbravation: Microchimica Acta

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Springer Vienna

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DOI

10.1007/bf00029156

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1436-5073

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Dynamic evaluation of cellsecreted interferon gam

Authors: Mingyao Zhu Yanjuan Tang Qingqing Wen Jiong Li Peihui Yang
Publish Date: 2016/03/07
Volume: 183, Issue: 5, Pages: 1739-1748
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Abstract

A sensitive electrochemiluminescent ECL immunosensor was developed for realtime and dynamic evaluation of the concentration of interferon gamma IFNγ that is secreted by peripheral blood mononuclear cells IFNγ is a diagnostic marker for latent infections with Mycobacterium tuberculosis MTB and its levels vary in response to drugs stimulation The ECL immunosensor was fabricated by attaching gold nanoparticlecoated magnetic beads AuNPMB on a nanofiber prepared from graphene oxide and polyaniline GOPANINF The sensor possesses a threedimensional structure nanocomposite that is beneficial for immobilization of primary antibodies Ab1 and this enhances the intensity of ECL The assay is based on a sandwichtype immunoreaction in which secondary antibodies Ab2 labeled with CdS quantum dots QDs are used to generate ECL The assay has a wide linear range 01–500 pg⋅mL−1 and a low detection limit 30 fg∙mL−1 The method was applied to realtime and dynamic determination of IFNγ in supernatants of peripheral blood mononuclear cells in response to stimulation by chlorogenic acid Conceivably it may be used for monitoring the dynamic variation of biomarkers in cells supernatants in response to drug stimulation and to diagnose other infectious diseasesAn electrochemiluminescent immunosensor was developed for realtime and dynamic evaluation of the concentration of interferon gamma IFNγ Analysis is based on the use of gold nanoparticlecoated magnetic beads AuNPMB that were attached to a nanofiber prepared from graphene oxide and polyaniline GOPANINF

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