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Title of Journal: Microchim Acta

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Abbravation: Microchimica Acta

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Springer-Verlag

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DOI

10.1016/0042-207x(86)90468-9

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1436-5073

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Flow Injection Chemiluminescence for the Determina

Authors: Shuhao Wang Lingyun Du Shilei Lin Huisheng Zhuang
Publish Date: 2006/05/11
Volume: 155, Issue: 3-4, Pages: 421-426
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Abstract

A novel approach to the detection of estriol using a flow injection system coupled to enhanced chemiluminescent immunoassay was developed based on noncompetitive immunoassay formats A conjugated estriolovalbumin immobilized immunoaffinity column was inserted into the flow system to trap the unbound horseradish peroxidase HRPlabeled antibody after an offline incubation of estriol and HRPlabeled antiestriol antibody The trapped enzyme conjugate was detected by the injection of chemiluminescent substrates to produce enhanced chemiluminescence The linear range for the determination of estriol is 100 to 400 ng · mL−1 with a correlation coefficient of 0996 and a detection limit of 50 ng · mL−1 The total time for sampling and chemiluminescent detection of one sample is 400 seconds after 30 min of preincubation The results for pregnancy serum samples obtained by this method are in good agreement with those obtained using ELISA

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  8. Ultrasensitive Assay of Rhein in Medicine Based on its Enhanced Luminol-K 3 Fe(CN) 6 Chemiluminescence Reaction Using the Flow Injection Technique
  9. Image Formation in Low Vacuum SEM
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