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Title of Journal: Plant Cell Tiss Organ Cult

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Abbravation: Plant Cell, Tissue and Organ Culture

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Kluwer Academic Publishers

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DOI

10.1007/bf02599264

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ISSN

1573-5044

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Indirect shoot organogenesis from leaves of Empha

Authors: Xiuli Shen Jianjun Chen Michael E Kane
Publish Date: 2007/06/07
Volume: 89, Issue: 2-3, Pages: 83-
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Abstract

A novel protocol for indirect shoot organogenesis of Dieffenbachia cv Camouflage was established using leaf explants excised from in vitro shoot cultures The frequency of callus formation reached 96 for explants cultured on Murashige and Skoog 1962 basal medium supplemented with 5 μM thidiazuron and 1 μM 24dichlorophenozyacetic acid The number of shoots regenerated was high with up to 79 shoots produced per callus cultured on basal medium supplemented with 40 μM N 6Δ2isopentenyladenine and 2 μM indole3acetic acid Regenerated shoots rooted well in a soilless substrate acclimatized ex vitro at 100 and grew vigorously under shaded greenhouse conditions Somaclonal variations in leaf variegation color and morphology have been observed in regenerated plants

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