Authors: E A Popowich A P Firsov T Y Mitiouchkina V L Filipenya S V Dolgov V N Reshetnikov
Publish Date: 2007/07/28
Volume: 90, Issue: 3, Pages: 237-244
Abstract
Transgenic plants of hyacinth Hyacinthus orientalis L cvs Edisson and Chine Pink have been obtained by Agrobacteriummediated transformation Leaf explants of the both hyacinth cultivars regenerated shoots on MS medium containing 22 μM BAP and 03 μM NAA at a frequency of 95 A tumefaciens strain CBE21 carrying binary vector pBIThau35 was used for transformation Plasmid pBIThau35 has been produced by cloning preprothaumatin II cDNA into pBI121 instead of uidA gene Inoculated leaf explants formed calli and shoots at high frequency on selective medium with 100 mg l−1 kanamycin Four hyacinth transgenic lines of cv Chine Pink and one line of cv Edisson have been selected on medium containing 200 mg l−1 kanamycin The insertion of thaumatin II gene into hyacinth genome has been confirmed by PCRanalysis All transgenic plants expressed substantial amounts of thaumatin II between 006 and 028 of the total soluble protein Hyacinth transgenic lines were assayed for resistance to the pathogenic fungi Fusarium culmorum and Botrytis cinerea There were no significant differences between nontransformed control and transgenic leaves of both cultivars At the same time the bulbs of the transgenic line Н7401 cv Chine Pink showed the higher level of resistance to B cinerea the bulbs of the transgenic line Н7404 were more resistant to F culmorum In both cases the signs of the fungal disease were developed more slowly The resistance of the bulbs cv Edisson line to these fungi was not changed All transgenic hyacinth plant were successfully transferred to soil for further evaluation
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