Authors: E Cosi P Bettini D Bindi M Buiatti
Publish Date: 2007/06/13
Volume: 89, Issue: 2-3, Pages: 203-209
Abstract
The production of reactive oxygen species ROS in plants is a common event in metabolic and physiological processes as well as in the response to biotic and abiotic stress In this paper we will report that root tissue from axenically grown tomato cultivars and Lycopersicon wild species can be used for the determination of superoxide production Superoxide generation was evaluated following the treatment of root tissues with two general elicitors of the defence response laminarin and calcium ionophore A23187 Results demonstrated that elicitor reactivity in terms of superoxide generation of the tomato cultivars and the wild species used was different This suggested varying levels of competence for nonspecific active defence The proposed technique merges the advantages of in vitro cultures and of whole tissues and also demonstrates that root tissue is a suitable material for evaluating free radical releaseThis Research was funded by the Italian Ministry of Agricultural and Forest Policies project ‘Plant protection using molecular markers PROMAR Authors are grateful to Prof A Scala Department of Agricultural Biotechnology University of Florence Italy for helpful discussion and critical reading of the manuscript to Prof FM Stefanini Department of Statistics ‘Giuseppe Parenti’ University of Florence Italy for critical discussion on statistical analysis and to Dr M Halperin for revising the English form of the manuscript
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