Selenium Deficiency Influences Nitric Oxide and Se

Authors: Xia Zhao Haidong Yao Ruifeng Fan Ziwei Zhang Shiwen Xu

Publish Date: 2014/10/16

Volume: 161, Issue: 3, Pages: 341-349

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Abstract

Selenium Se deficiency induces pancreatic atrophy in chickens but the molecular mechanism remains unclear In this study we investigated the effect of dietary Se deficiency on the expressions of 25 selenoproteins and the content of nitric oxide NO and examined the relationship between selenoproteins and NO Chickens 180 1 day old were randomly divided into two groups low L group fed with Se deficient Se 0033 mg/kg diet and control C group fed with normal Se 02 mg/kg diet Then pancreas was collected at 15 25 35 45 and 55 days and the content of NO the activity of inducible NO synthase iNOS and the messenger RNA mRNA levels of 25 selenoproteins and iNOS were measured The results showed that 25 selenoproteins were decreased P  005 by Se deficiency Among them thioredoxin reductase 1 TXNRD1 selenoprotein S SELS selenoprotein U SELU selenoprotein X1 SEPX1 and selenoprotein synthetase 2 SPS2 were highly and extensively expressed than other types of selenoproteins in pancreas of chickens P  005 Thioredoxin reductase 2 TXNRD2 glutathione peroxidase 1 GPX1 glutathione peroxidase 3 GPX3 selenoprotein I SELI iodothyronine deiodinase 1 DIO1 selenoprotein P1 SEPP1 selenoprotein W1 SEPW1 selenoprotein O SELO selenoprotein T SELT selenoprotein M SELM selenoprotein X1 SEPX1 and SPS2 were excessively decreased P  005 Meanwhile NO content iNOS activity and mRNA level were increased strikingly compared with C group P  005 The correlation analysis suggested that NO had a strong negative correlation with GPX1 glutathione peroxidase 2 GPX2 GPX3 DIO1 selenoprotein K SELK SELI SEPX1 and SPS2 These results suggested that Se deficiency induced pancreatic injury by influencing NO and selenoproteins in pancreas of chickens Thus it offers some information on the mechanism of pancreatic injury induced by Se deficiencyThis study was supported by the National Natural Science Foundation of China 31272626 the Study Abroad Foundation of Heilongjiang Province LC201031 the Doctoral Fund of the Ministry of Education of China 20122325110018 and the Heilongjiang Postdoctoral Fund LBHZ13028

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