Functional Improvement in Rats’ Pancreatic Islets

Authors: Shermineh MoeiniNodeh Mahban Rahimifard Maryam Baeeri Mohammad Abdollahi

Publish Date: 2016/05/27

Volume: 175, Issue: 1, Pages: 146-155

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Abstract

According to undiscovered toxicity and safety of magnesium oxide nanoparticles MgO NPs in isolated pancreatic islet cells this study was designed to examine the effects of its various concentrations on a timecourse basis on the oxidative stress viability and function of isolated islets of rat’s pancreas Pancreatic islets were isolated and exposed to different MgO NP 100 nm concentrations within three different time points After that oxidative stress biomarkers were investigated and the best exposure time was selected Then safety of MgO NPs was investigated by flow cytometry and fluorescent staining and levels of insulin secretion and caspase activity were measured The results illustrated a considerable decrease in oxidative stress markers such as reactive oxygen species ROS and lipid peroxidation LPO levels of pancreatic islets which were treated by MgO NPs for 24 h Also in that time of exposure cell apoptosis investigation by flow cytometry and insulin test showed that MgO NPs in a concentration of 100 μg/ml decreased the rate of apoptotic cells via inhibiting caspase9 activity and made a significant increase in the level of insulin secretion Data of function and apoptosis biomarkers correlated with each other It is concluded that the use of MgO NPs in concentration of as low as 100 μg/ml can induce antiapoptotic antioxidative and antidiabetic effects in rat pancreatic islets which support its possible benefit in islet transplantation procedures

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