Analysis of Ionomic Profiles of Canine Hairs Expos

Authors: KyoungMin So Yoonseok Lee Jin Duck Bok Eun Bae Kim Myung Il Chung

Publish Date: 2016/01/13

Volume: 172, Issue: 2, Pages: 364-371

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Abstract

The purpose of this study was to provide a new insight on the response of canines to stress exposure the ionomic profiles of canine hair 28 ± 03 years 1517 ± 21 kg n = 10 was determined before and after lipopolysaccharide LPS injections LPS was intramuscularly injected to induce inflammatory stress responses which were confirmed by observing increases in the level of serum cortisol aldosterone and inflammatory cytokines such as IL6 IL1β and TNFα The hair contents of 17 elements were obtained by applying analytical procedures using the inductively coupled plasma mass spectrometry ICPMS The following elements sodiumNa and potassiumK among macroelements ironFe and manganeseMn among microelements and aluminumAl nickelNi and leadPb for toxic elements showed significant increased levels with the immunological stress The degree of increase in toxic elements was remarkable with the stress exposure A fortyfivefold increase seen in Al accumulation with the stress exposure was noteworthy Although mercuryHg and cadmiumCd showed decreased levels with the stress exposure the degree was negligible compared to the level of increase Correlation pattern between the elements was changed with the immunological stress Toxic elements became more correlated with macro or microelements than with toxic elements themselves after the stress exposure Principal component analysis PCA showed that LPS challenge shifted the overall hair mineral profiles to a consistent direction changing Al and K up even in animals with different hair mineral profiles before LPS treatment In conclusion the multivariate data processing and study of element distribution patterns provided new information about the ionomic response of the canine hairs to immunological stress ie the ionomic profiles of canine hairs is strongly affected by the stress induced by LPS injectionsThis work was carried out with the support of “Cooperative Research Program for Agriculture Science Technology Development Project title Development of screening and management technique for health status of detector dogs using noninvasive tools Project No 00957703” Rural Development Administration Republic of Korea

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