Acute Cadmium Exposure Reduces the Local Angiotens

Authors: Gilson B BroseghiniFilho Camila C Pereira Almenara Marcus V A Vescovi Thaís de O Faria Dalton V Vassallo Jhuli K Angeli Alessandra S Padilha

Publish Date: 2015/02/10

Volume: 166, Issue: 2, Pages: 149-156

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Abstract

Cadmium exposure causes health problems that may result from increased oxidative stress and from changes in enzyme metalloproteases activities as angiotensinconverting enzyme ACE In fact cadmium produces inhibition of serum ACE but is not known how cadmium acts on tissue ACE activity and whether following acute exposure tissue cadmium content is increased In order to elucidate these issues a cadmium bolus was injected intravenously in Wistar rats and the cadmium content and the ACE activity were measured in the serum lungs aorta and kidneys Moreover in order to clarify if the cadmium affects directly tissue ACE activity acute metal exposure in vitro was performed Our results demonstrated that 120 min following cadmium administration blood and organ cadmium content were both increased Serum and lung ACE activity were reduced following acute cadmium exposure but aortic and kidney ACE activities were not affected The inhibitory effects induced by cadmium on ACE activity were also observed in the serum as well as the lungs and the aorta but not in the kidneys following in vitro exposure Moreover the inhibitory effects induced by cadmium on ACE activity were partially restored in vitro by zinc supplementation suggesting a possible interaction or competition between cadmium and zinc by at the active site of ACE Summarising our results suggest that acute cadmium exposure promotes an increase in the tissue metal content that was accompanied by direct inhibition of serum aorta and lung ACE activity an effect that is cadmium concentrationdependent and is partially reversed by zincThis project was supported by CAPES Coordenação de Aperfeiçoamento de Pessoal de Nível Superior and CNPq/FAPES Conselho Nacional de Desenvolvimento Científico e Tecnológico and Fundação de Amparo à Pesquisa do Espírito Santo 54668662/2011 The funders had no role in the study’s design data collection or analysis nor were they involved in the manuscript’s preparation or the decision to publish it

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