Quercetin and Vitamin C Mitigate Cobalt ChlorideI

Authors: Temitayo Olabisi Ajibade Ademola Adetokunbo Oyagbemi Temidayo Olutayo Omobowale Ebunoluwa Racheal Asenuga Kabirat Oluwaseun Adigun

Publish Date: 2016/06/10

Volume: 175, Issue: 2, Pages: 347-359

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Abstract

The objective of the present work was to evaluate the toxic effects of cobalt chloride a potent oxidative stressinducing chemical at 650 ppm in rats and the protective effect of quercetin and/or vitamin C against the cobalt chlorideinduced toxicity Thirty rats were randomly selected and assigned to one of five groups control cobalt chloride cobalt chloride + quercetin cobalt chloride + vitamin C and cobalt chloride + quercetin + vitamin C The exposure of rats to cobalt chloride led to a significant increase p  005 in malondialdehyde MDA and hydrogen peroxide H2O2 generated but decreased nitric oxide NO bioavailability Also significant p  005 reductions were observed in the activity of glutathione peroxidase GPx and reduced glutathione GSH content in the cardiac and renal tissues Treatment with quercetin and vitamin C reversed the effect of cobalt chloride on MDA H2O2 and NO more potently than with either of the two antioxidants and increased the antioxidant defence system Further treatment of rats with quercetin and vitamin C in combination resulted in significant p  005 decreases in the systolic diastolic and mean arterial blood pressure of rats relative to those exposed to cobalt chloride alone Immunohistochemical studies revealed a greater expression of nuclear factor kappa beta NFkB in the cobalt chloride group compared with the control and antioxidantstreated rats The results of this study suggest a protective role for quercetin and vitamin C in the amelioration of the toxic mechanisms leading to cobalt chlorideinduced hypertension and its associated cardiac and renal complications in rats

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